火龙果根系在淹水胁迫下的基因差异表达

Differential Gene Expression of Pitaya Root Induced Under Flooding Stress

运用RNA-Seq测序技术对淹水胁迫下的火龙果根系进行转录组测序,对淹水处理组与对照组的基因表达量进行差异比较分析,并采用qRT-PCR对差异表达基因的表达特性进行分析验证,结果表明:淹水胁迫下火龙果根系中有1 481个差异表达的基因,其中,上调表达925个,下调表达556个,其功能多富集于糖代谢、碳代谢、氮代谢、乙醇代谢、氨基酸生物合成、苯丙氨酸代谢、植物激素信号转导等方面。可见,淹水胁迫对火龙果根系的基础代谢影响较大,但火龙果根系能够通过糖酵解、乙醇发酵及丙氨酸发酵途径来提供火龙果植株生存所需能量以减轻淹水胁迫对其造成的不良影响,并能够诱导乙烯合成来调控火龙果的逆境应答,从而使火龙果植株表现出一定的淹水耐受性。

The RNA-Seq sequencing technique was used to sequence the root system of pitaya under flooding stress, and the difference of gene expression between the flooded group and the control group was compared. The expression characteristics of the differentially expressed genes were analyzed and verified by qRT-PCR to explore the molecular mechanism of flooding stress and explore genes related to flooding tolerance. The results showed that there were 1 481 differentially expressed genes in the root system of pitaya under flooding stress, including 925 up-regulated genes and 556 down-regulated genes. Their functions were mainly enriched in sugar metabo- lism, carbon metabolism, nitrogen metabolism, ethanol metabolism, amino acid biosynthesis, phenylalanine me- tabolism, plant hormone signal transduction and so on. It can be seen that flooding stress has a great effect on the basic metabolism of pitaya root, but the root system of pitaya can provide the energy required for the survival of pitaya through glycolysis, ethanol fermentation and alanine fermentation to reduce the adverse effects of flood- ing stress on pitaya. And flooding stress induced ethylene synthesis to regulate the stress response of pitaya so that the pitaya showed a certain tolerance to flooding stress.