毛细管超速离心法在输血后患者Rh血型鉴定中的应用分析

Application of Capillary Ultracentrifugation in Identification of Rh Blood Group in the Patients after Transfusion

目的探讨毛细管超速离心技术在输血后患者Rh血型鉴定中的应用。方法对送检的10例抗体筛选阳性、Rh抗原鉴定为混合视野的患者红细胞,洗涤制备压积红细胞,应用毛细管超速离心技术进行分离,分别取近心端和远心端红细胞进行Rh血型鉴定。结果 10例患者标本经毛细管离心分离后,均能准确鉴定Rh血型,而且可以辅助鉴定抗体类型。结论毛细管超速离心技术可较好地分离输血后患者红细胞,进而准确鉴定患者血型,值得在临床输血中推广使用。

Objective To investigate the feasibility of capillary ultracentrifugation for identification of Rh bloodgroup in patients after transfusion. Methods Erythrocytes of 10 patients with positive antibody screening and Rh antigenconfirmed in the mixed field of view were washed to prepare pressed red blood cells. The blood cells were isolated by capillaryultracentrifugation. The Rh blood group was identified using both proximal and distal red blood cells. Results Ten specimensfrom the patients after capillary centrifugal separationwere accurately identified as Rh blood group,with its capability ofidentifying antibody types.Conclusion Capillary ultracentrifugation technique can isolate the red blood cells of patientsfollowing transfusion and correctly indicate the blood type of patients,being applicable to clinical blood transfusion.