电针对高脂饮食诱导的肥胖大鼠白色脂肪组织中白介素-6、波形纤维蛋白的影响

Electroacupuncture Reduces Body Fat by Down-regulating IL-6 and Vimentin in Abdominal White Adipose Tissue of Diet-induced Obesity Rats

目的:观察电针对高脂饮食诱导的肥胖(DIO)大鼠白色脂肪组织(WAT)中白介素-6(IL-6)和波形纤维蛋白(vimentin)的影响,探讨针灸减肥的作用机制。方法:清洁级3月龄雄性Wistar大鼠随机分为对照组(10只)和模型组、电针组、假电针组(每组8只)。以高脂饲料喂养建立营养型肥胖动物模型。电针组电针"足三里""天枢"穴,疏密波,频率2Hz/15Hz,强度1mA,假电针组取"足三里""天枢"穴旁开5mm区域的非穴位区作为针刺点,不加电针,2组均30min/次,5次/周,共8周。治疗期间,对照组、模型组不做任何处理,所有大鼠均饲喂普通饲料,各组大鼠体质量每周测量1次。HE染色法观察WAT中脂肪细胞的形态学改变;蛋白免疫印迹法检测大鼠WAT中IL-6、vimentin蛋白含量。结果:治疗后,与对照组比较,模型组大鼠体质量显著升高(P<0.05);与假电针组和模型组比较,电针组大鼠体质量显著降低(P<0.05)。与对照组比较,模型组大鼠WAT的脂肪细胞直径显著增大(P<0.05);与模型组、假电针组比较,电针组大鼠WAT的脂肪细胞直径显著减小(P<0.05)。与对照组比较,模型组大鼠WAT中IL-6、vimentin蛋白含量显著升高(P<0.05);与假电针组和模型组比较,电针组大鼠WAT中IL-6、vimentin含量显著降低(P<0.05)。结论:电针能有效下调DIO大鼠WAT中IL-6、vimentin的表达,可能是电针通过调节肥胖模型慢性炎性反应以减轻体脂的效应机制之一。

Objective To investigate the effect of electroacupuncture（EA）on IL-6 and vimentin protein expression in white adipose tissue（WAT）of diet-induced obesity（DIO）rats,so as to reveal its mechanism underlying losing weight.Methods Thirty-four Wistar rats were randomly divided into normal（control,n=10）,model,sham-EA and EA groups（n=8 in each of the latter 3 groups）.The obesity model was established by feeding the rats with high fat diet.EA（2 Hz/15 Hz,1 mA）was applied to bilateral＂Zusanli＂（ST 36）and＂Tianshu＂（ST 25）for 30 min,5 times per week for a total of 8 weeks.For sham-EA group,two sham points（about 5 mm lateral to ST 36 and to ST 25）were only punctured with filiform needles but without electrical stimulation.During EA treatment,all rats were fed with normal fodder,and their body weight were measured once a week.Histopathologic changes（diameters of adipose cells）of abdominal WAT were observed under microscope after sectioning and H.E.staining,and the expression levels of IL-6 and vimentin in the WAT were detected by Western blot.Results Compared with the control group,the body weight,diameter of fat cells and the expression levels of IL-6 and vimentin in the WAT were significantly increased in the model group（P〈0.05）.Following EA,the body weight,diameter of fat cells and the expression levels of IL-6 and vimentin proteins were considerably down-regulated in the EA group（P〈0.05）,rather than in the sham-EA group relevant to the model group（P〈0.05）.Conclusion EA intervention can effectively down-regulate the expression of IL-6 and vimentin in WAT of DIO rats,which may contribute to its action in reducing body fat by relieving chronic inflammation.