电针单穴与腧穴配伍对失眠大鼠血清中白介素-1β、脑源性神经生长因子等睡眠-觉醒相关因子含量的影响

Electroacupuncture of Multiple Acupoints Is Significantly Superior to That of Single Acupoint in Improving Sleep by Regulating Serum Sleep-promoting and Awakening-promoting Factors in Insomnia Rats

目的:通过观察电针刺激单穴与腧穴配伍对对氯苯丙氨酸(PCPA)致失眠大鼠血清内源性睡眠-觉醒调节相关因子白介素-1β(IL-1β)、脑源性神经生长因子(BDNF)、前列腺素D2(PGD2)、褪黑激素(MLT)和皮质酮(CORT)含量的影响,探讨电针单穴与腧穴配伍对失眠的调节效应差异及其作用机制。方法:SD大鼠随机分为正常组、模型组、神门组、百会组、三阴交组、神门-百会-三阴交组(配伍组),每组9只。采用连续2d腹腔注射PCPA悬浊液(300 mg/kg)建立失眠大鼠模型,各治疗组分别电针"百会"或双侧"神门""三阴交""神门+百会+三阴交"穴,每次30min,1次/d,连续4d。治疗后比较各组大鼠睡眠潜伏期(SL)和睡眠时间(ST);ELISA法检测血清IL-1β、BDNF、MLT、PGD2和CORT的含量。结果:与正常组相比,模型组SL明显延长(P<0.01),ST显著缩短(P<0.01);与模型组比较,神门组、百会组与配伍组的SL均显著缩短(P<0.05,P<0.01),各单穴组与配伍组ST明显延长(P<0.05,P<0.01);与各单穴组相比,配伍组大鼠的SL明显缩短、ST显著延长(P<0.05)。与正常组相比,模型组血清IL-1β、BDNF、PGD2和MLT的含量显著降低(P<0.05),CORT的含量显著升高(P<0.05);与模型组相比,单穴组和配伍组血清IL-1β、BDNF、PGD2和MLT的含量显著升高(P<0.05),CORT的含量明显降低(P<0.05);配伍组IL-1β、BDNF、PGD2的含量显著高于百会组和三阴交组(P<0.05),MLT的浓度显著高于所有单穴组(P<0.05),CORT的浓度明显低于神门组和三阴交组(P<0.05)。结论:电针单穴"神门""百会"或"三阴交"和腧穴配伍均可改善大鼠的睡眠状况,此效应可能与提高血清中IL-1β等促睡眠因子和下调促觉醒因子CORT的含量有关,并且腧穴配伍应用的调节作用总体上要优于单穴。

Objective To observe the effect of electroacupuncture（EA）stimulation of single and multiple acupoints on sleep and concentrations of interlukin-1β（IL-1β）,brain-derived neurotrophic factor（BDNF）,prostaglandin D2（PGD2）and melatonin（MLT,sleep-promoting factors）and corticosterone（CORT,awakening-promoting factor）in the serum in insomnia rats,so as to explore its efficacy difference and the mechanism underlying improving sleep.Methods Fifty-four male SD rats were randomly divided into control,model,EA-Baihui（GV 20）,EA-Shenmen（HT 7）,EA-Sanyinjiao（SP 6）and EA-GV 20＋HT 7＋SP 6 groups（n=9 rats in each group）.The insomnia model was established by intraperitoneal injection of para-chlorophenylalanine（PCPA,300 mg/kg）once daily for 2 days.In the EA-GV 20,EA-HT 7,EA-SP 6 and EA-GV 20＋HT 7＋SP 6 groups,EA stimulation was administrated for 30 min,once a day for 4 days.The sleep onset latency and sleep duration were measured after intraperitoneal injection of pentobarbital sodium（35 mg/kg）.The concentrations of IL-1β,BDNF,MLT,PGD2 and CORT in the serum were detected by ELISA.Results After EA stimulation of GV 20,HT 7,SP 6 and GV 20＋HT 7＋SP 6,the sleep latency was significantly shortened（P〈0.05,P〈0.01,except SP 6）,and the sleep duration was remarkably prolonged in comparison with the model group（P〈0.05,P〈0.01）,and the therapeutic effects of EA-GV 20＋HT 7＋SP 6 were significantly superior to those of EA-GV 20,EA-HT 7 and EA-SP 6 in shortening the sleep latency and lengthening the sleep duration（P〈0.05）.Following modeling,the concentrations of IL-1β,BDNF,PGD2 and MLT were significantly down-regulated,and the CORT level was markedly upregulated in the model group relevant to the control group（P〈0.05）.Following EA,modeling induced dramatic decrease of serum IL-1β,BDNF,PGD2 and MLT was considerably up-regulated,and the increased CORT level markedly down-regulated in the EA-GV 20,EA-HT 7,EA-SP 6 and EA-GV 20＋HT 7＋SP 6 groups（P〈0.05）.The effects of EA-GV 20＋HT 7＋SP 6 were evidently superior to those of EA-GV 20 and EA-SP 6 in up-regulating serum IL-1β,BDNF and PGD2 levels,and to those of HT 7,GV20 and SP 6 in up-regulating serum MLT level,and significantly superior to those of EA-ST 7 and EA-SP 6 in down-regulating serum CORT（P〈0.05）.Conclusion EA stimulation of HT 7,GV 20,SP 6 and GV 20＋HT 7＋ SP 6 can significantly improve the sleep in insomnia rats,which is closely associated with its effects in regulating serum sleep-promoting factors and awakeningpromoting factor.Joint administration of EA of GV 20＋HT 7＋ SP 6 has a better effect than the single acupoint mentioned above.