微流控技术制备ZnO纳米棒及生物荧光检测性能研究

Fabrication and Fluorescence Biodetection of ZnO Nanorods Using Microfluidic Technology

本研究设计并制备了一种微流控芯片并在其中水热合成了氧化锌(ZnO)纳米棒。利用扫描电子显微镜(SEM)和X射线衍射(XRD)研究了合成条件对ZnO纳米棒的形貌和晶体结构的影响。结果表明,在微流控芯片中可制备得到致密的ZnO纳米棒,其直径和长度随加热方式和制备时间的变化而改变。对比研究不同加热方式制备的ZnO纳米棒阵列检测异硫氰酸荧光素标记的羊抗牛IgG的性能,发现局部加热方式制备的ZnO纳米棒检测荧光素标记蛋白的性能更佳,在10pg/mL～1?g/mL范围内线性良好,相关系数为0.99209。在此基础上,用局部加热制备的ZnO纳米棒检测人甲胎蛋白(AFP),其最低检测限可达1pg/mL。这些结果表明,微通道中合成的ZnO纳米棒适用于多通道荧光检测。

A simple microfluidic chip was designed and fabricated, on which zinc oxide （ZnO） nanorods were syn- thesized by hydrothermal method. The influence of growth condition on morphology and crystal structure of ZnO nanorods were investigated by scanning electron microscope （SEM） and X-ray diffraction （XRD）, respectively. Ex- perimental results showed that densely packed ZnO nanorods could be prepared on microfluidic chip, with good crys- tallinity and c-axis orientation. The diameter and length of ZnO nanorods changed with heating methods and growth time. Moreover, fluorescence detection by ZnO nanorods, synthesized with different heating methods, were evalu-ited by FITC-labeled goat anti-bovine IgG. And the results revealed that ZnO nanorods synthesized on chip by localized heating showed better detection performance of fluorescein-labeled protein and good linearity from 10 pg/mL to 1 μg/mL with the correlation coefficient of 0.99209. The as-synthesized ZnO nanorods were further used to demon- strate detection of human α-fetoprotein （AFP）, and achieved a superior limit of detection as low as 1 pg/mL. This work demonstrated that ZnO nanorods synthesized by micro fluidic device allowed multiplexed fluorescence biodetection.