摘要
从猪、牛、鸡、鸭和鲨鱼软骨中提取分离硫酸软骨素,采用酶解-高效液相色谱法对不同来源和制备工艺获得的硫酸软骨素进行了二糖分析。结果表明,来源于猪、牛、鸡和鸭软骨的硫酸软骨素由非硫酸化二糖、4-硫酸化二糖和6-硫酸化二糖组成,而来源于鲨鱼软骨的硫酸软骨素还含有2, 6-硫酸化二糖、2, 4-硫酸化二糖和4, 6-硫酸化二糖,且各二糖单位的百分含量也不同;高温、强酸、强碱及双氧水的强氧化性均可降低硫酸软骨素中硫酸化二糖的百分含量。说明硫酸软骨素的组成与其来源和制备工艺密切相关,实际生产中可根据不同需求来选择合适的原料和制备工艺。
Chondroitin sulfates were extracted and isolated from bovine, porcine, chicken, duck and shark cartilage. Components analysis of chondroitin sulfate from different origins and process conditions by high performance liquid chromatography after enzymatic hydrolysis was studied. The results showed that chondroitin sulfate from bovine, porcine, chicken and duck cartilage are constituted of different percentages of nonsulfated disaccharide, disaccharide 4-sulfate and disaccharide 6-sulfate. In addition to the above disaccharides, chondroitin sulfate fi＇om shark cartilage also contains disaccharide 2, 6-sulfate, disaccharide 2, 4-sulfate and disaccharide 4, 6-sulfate. Results also suggested that the percentages of sulfated disaccharides of chondroitin sulfate reduced under high temperature or strong acidic or alaline or oxidable conditions. So component of chondroitin sulfate was closely related to its origins and process conditions. And we should choose the appropriate materials and process conditions according to our requirements in practical production.
作者
罗小芳
郭乙颖
陈海峰
秦源
宁佳前
王宗成
LUO Xiaofang1,2, GUO Yiying1, CHEN Haifeng1, QIN Yuan1, NING Jiaqian1, WANG Zongcheng1,2(1. Hunan University of Science and Engineering, College of Chemical and Biological Engineering （Yongzhou 425199）; 2. Key Laboratory of Comprehensive Utilization of Advantage Plants Resources of Hunan （Yongzhou 425199）)
出处
《食品工业》
CAS
北大核心
2018年第10期1-5,共5页
The Food Industry
基金
湖南科技学院湘南优势植物资源综合利用湖南省重点实验室开放基金资助(XNZW15C04)
湖南省科技厅重点研发计划项目(2016NK2098)
