食蟹猴肺上皮细胞株MFLE-001的建立及生物学特性研究

Establishment and Biological Characteristics of Lung Epithelial Cell Line MFLE-001 from Macaca fascicularis

该研究建立了食蟹猴肺上皮细胞株并鉴定其生物学特性。以食蟹猴肺部组织进行原代培养,用差速贴壁法纯化细胞株,并通过倒置显微镜、透射电镜、扫描电镜观察,绘制生长曲线,进行核型分析以及肺上皮标志物检测等方法研究其生物学特性。结果显示,体外培养细胞生长均一、稳定,细胞倍增时间为4.8天,免疫组化CK(+)、CK-7(+)、波形蛋白(+),染色体分析表明,具有正常2倍染色体数目(2n=42),符合食蟹猴肺上皮细胞株特性。该研究成功建立第一株食蟹猴肺上皮细胞株,命名为MFLE-001,为肺部疾病研究和药物筛选提供了理想的体外实验模型。

In the present study, the lung epithelial cell line from Macaca fascicularis was established and its biological characteristics was identified. The cells derived from a pulmonary tissue of Macaca fascicularis were primarily cultured and purified by differential attachment technique. The biological characteristics of the cell line were studied by using optical microscope, electron microscopes, growth curve, karyotype analysis and lung epithelial marker. The results showed that the growth of cells were stable and uniform in vitro. The cell doubling time was 4.8d and the results of immunohistochemistry showed the expressions of CK and CK-7. The vimentin was positive and the chromosome numbers were 2n=42, all of which were accorded with the characteristics of lung epithelial cell line from Macaca fascicularis. MFLE-001 has been proved to be the first lung epithelial cell line of Macaca fascicularis and is an ideal experimental model for lung disease research and drug screening.