背部推拿法对亚急性衰老并免疫功能低下家兔局部皮肤与脑组织IL-33表达的影响

Infl uence study of Tuina therapy on the back on the expression of IL-33 in the skin and brain of subacute aging and immunocompromised rabbits

目的观察对亚急性衰老并免疫功能低下家兔开展背部推拿法后机体局部皮肤与脑组织白介素-33(IL-33)蛋白表达的变化情况。方法将28只家兔随机平均分为对照组、模型组、推拿组和药物组,除对照组外,其余各组均采用D-半乳糖腹腔注射形成亚急性衰老模型的方法造模。推拿组采用背部推拿法干预,药物组采用口服黄芪精口服液干预。各组均观察局部皮肤与脑组织IL-33蛋白表达的变化情况。结果模型组与空白组比较,家兔局部皮肤中IL-33表达减少,差异具有统计学意义(P <0.05)。推拿组和药物组与模型组比较,家兔局部皮肤与脑组织IL-33蛋白表达增强,差异具有统计学意义(P <0.05)。药物组与推拿组比较,家兔局部皮肤与脑组织中IL-33蛋白表达无显著性差异,但推拿组局部皮肤IL-33蛋白表达均数略高于药物组。结论背部推拿法能够有效提高机体局部免疫功能,其可能机制之一是背部推拿法通过提高机体局部皮肤组织与脑组织IL-33含量来实现免疫调节功能的。

Objective To observe the changes of protein expression of IL-33 in the skin and brain of subacute aging and immunocompromised rabbits after tuina therapy on the back. Methods Twenty-eight rabbits were randomly divided into blank group, model group, tuina group and drug group. Except for the control group, the peritoneal injection of D-galactose was used to prepare the immunosuppressive rabbit. The tuina group was treated by tuina therapy on the back, and the drug group was treated with Astragalus oral liquid. The protein expression of IL-33 protein in the skin and brain tissues was observed in each group. Results Compared with the blank group, the expression of IL-33 in the rabbit skin and brain was decreased in the model group and the difference was statistically signifi cant（P〈0.05）.Compared with the model group,the expression of IL-33 in the local skin and brain of the rabbit in the tuina group and drug group was increased and the difference was statistically signifi cant（P〈0.05）. Compared with the tuina group, there was no signifi cant difference in the expression of IL-33 in the skin and brain of rabbits, but the mean number of expression of IL-33 in the skin in the tuina group was slightly higher than that in the drug group. Conclusion Conclusion The tuina therapy on the back can effectively improve the body＇s local immune function; one of the possible mechanisms is that the tuina therapy on the back can improve the immune regulation function by increasing the IL-33 content of the skin tissue and brain tissue.