龙胆中当药苷和獐牙菜苦苷的含量测定及指纹图谱研究

Study on content determination and fingerprint of gentiana swertiamarin and sweroside

目的建立同时测定吉林省不同产地、不同药用部位龙胆中当药苷和獐牙菜苦苷含量的HPLC分析方法及龙胆药材的指纹图谱。研究龙胆不同产地、不同药用部位成分差异并加以区别。方法采用高效液相色谱法测定龙胆中当药苷和獐牙菜苦苷的含量,同时采用HPLC梯度洗脱技术建立指纹图谱系统,并进行聚类分析。结果当药苷和獐牙菜苦苷分别在0.1~0.35μg(r=0.999 9)、0.19~0.68μg(r=0.999 9)内呈良好的线性关系,平均加样回收率为97.40%、96.63%,RSD值分别为1.31%、1.21%。左家龙胆根中当药苷及獐牙菜苦苷的含量较高。结论龙胆中獐牙菜苦苷的含量较高,而当药苷的含量稍低。本研究将为后续龙胆的应用与开发提供理论基础。

Objective To establish the HPLC method for the simultaneous determination of chiratin and swertiamarin gentian from different areas of Jilin Province and to establish the fi ngerprints of radix gentiana. Study of different origin, different parts of the gentian medicinal differences in composition and to be distinguished.Methods HPLC were used to determine the content of chiratin and swertiamarin in Gentian. At the same time, the fi ngerprinting system was established by HPLC gradient elution technique and clustering analysis was carried out.Results The results showed that there was a good linear relationship between the chiratin and swertiamar in the range of 0.1 ～ 0.35μg（r = 0.9999） and 0.19 ～ 0.68 μg（r = 0.9999） respectively. The average recoveries were 97.40%and 96.63%, RSD values were 1.31% and 1.21% respectively. Left home gentian root contain lots of chiratin and swertiamar. Conclusion The contents of swertiamarin in gentian are higher, while the content of chiratin is lower. This study will provide the theoretical basis for the application and development of gentian.