AFP通过激活STAT3的磷酸化促进肝癌细胞的增殖

AFP Promotes Proliferation of Hepatocellular Carcinoma Cells by Activating Phosphorylation of STAT3

[目的]研究AFP基因过表达对肝癌细胞系Hep G2细胞增殖的影响及其机制。[方法]利用慢病毒转染技术对Hep G2细胞进行AFP基因过表达,通过Western Blot检测转染后Hep G2细胞中AFP表达情况,采用流式细胞技术和CCK8细胞增殖实验检测细胞的增殖情况,Western Blot检测AFP过表达后STAT3、p-STAT3蛋白水平的变化。[结果]CCK8细胞增殖实验显示,AFP过表达组Hep G2细胞较对照组细胞增殖速度快（P〈0.05）。流式细胞实验显示,AFP基因过表达组处于G1期和S期的细胞占比分别为49%和21%,而对照组相应的G1期、S期细胞占比分别为61%和12%,差异具有统计学意义（P〈0.05）。AFP基因过表达后,Hep G2细胞中p-STAT3表达水平升高,表明存在STAT3蛋白的激活。[结论]AFP可促进Hep G2肝癌细胞的增殖,其机制涉及STAT3蛋白的激活。

[Purpose] To investigate the effect of AFP on the proliferation of hepatocellular carcinoma cells（HCC）. [Methods] Lentivirus-mediated recombination AFP was transfected into HCC Hep G2 cells. Western Blot was used to detect the efficiency of AFP transfection. The cell proliferation and cell cycle were detected by CCK8 assay and flow cytometry,respectively. The protein level of AFP,STAT3 and p-STAT3 were detected by Western blot. [Results] The CCK8 assay indicated that overexpression of AFP significantly enhanced the proliferation of Hep G2 cells comparing to the negative control（P0.05）. G1 and S phase of Hep G2 cells in AFP over-expressed Hep G2 cells accounted for 49% and 21%,while those in the negative control group accounted for 61% and12%,respectively（P0.05）. The expression of p-STAT3 was increased in APF over-expressed Hep G2 cells. [Conclusion] AFP can promote proliferation of Hep G2 cells by activating phosphorylation of STAT3.