少阳生骨方对SD大鼠胫骨骨折断端18F-NaF分布的影响

Effects of ShaoYangShengGuFang Prescription on the distribution of 18F-NaF in rat tibial fracture bone

目的探讨^18F-NaF在评价骨折早期愈合方面的应用价值,以及少阳生骨方对大鼠骨折愈合的影响。方法将72只SD大鼠进行左侧胫骨骨折造模,造模后随机分为实验组（少阳生骨方汤剂灌胃）、对照组（骨肽片悬浮液灌胃）和空白组（0.9%生理盐水灌胃）,每组24只。在7、15、30、56 d从3组中各随机选取6只大鼠,对胫骨骨折断端进行PET/CT扫描,计算骨折断端对^18F-NaF的摄取。结果实验组和对照组^18F-NaF摄取值于15 d达到峰值（9.00±0.42）%ID/g、（8.10±0.71）%ID/g,而空白组^18F-NaF摄取值于30 d达到峰值（4.25±0.35）%ID/g,且在四个不同时间点,实验组摄取量与对照组间差异无统计学意义（P〉0.05）;实验组和对照组较空白组摄取量高,差异有统计学意义（P〈0.05）。结论^18F-NaF在评估早期骨折愈合中具有一定的应用价值,少阳生骨方能够促进大鼠胫骨骨折愈合。

Objective To explore the application value of ^18F-NaF in evaluation of early fracture healing, and the effects of ShaoYangShengGuFang Prescription on rat fracture healing. Methods 72 SD rats were performed left tibial fracture modeling. The model rats were randomly divided into experimental group（intragastric administration of ShaoYangShengGuFang Prescription）,control group（intragastric administration of Ossotide Tablets） and blank group（intragastric administration of 0.9% saline）. Every group included 24 rats. After 7, 15, 30, and 56 days, 6 rats were randomly selected from each of the three groups.The PET/CT scan was performed on the fractured ends of tibial fractures, and the uptake of ^18F-NaF at the fracture end was calculated. Results The ^18F-NaF uptake of experimental group and control group reached their peaks at the 15 th day（9.00±0.42 %ID/g、8.10±0.71 %ID/g）, while the ^18F-NaF uptake of blank group reached its peak at the 30 th day（4.25 ± 0.35 % ID/g）. At four different time points, there was no significant difference between the uptake of the experimental group and that of the control group in the ^18F-NaF uptake of rat tibial fracture bone（P〈0.05）, while the ^18F-NaF uptake of the experimental group or the control group was higher than that of the blank group, which was of remarkable statistical difference（P〈0.05）. Conclution ^18FNaF has some values in evaluating early fracture healing, and ShaoYangShengGuFang Prescription can promote the healing of tibial fractures in rats.