亚低温对大鼠脑缺血再灌注损伤后谷氨酸载体EAAC1 mRNA表达和损伤神经细胞凋亡的影响

Effects of mild hypothermia on the glutamate transporter EAAC1mRNA expression and injuried nervous cell apoptosis after rat focal cerebral ischemia/reperfusion injury

目的 探讨亚低温对大鼠脑缺血再灌注损伤后谷氨酸载体EAAC1mRNA表达和损伤神经细胞凋亡的影响。方法 采用大鼠局灶性脑缺血再灌注损伤模型 ,大脑中动脉阻塞 30min ,再灌注损伤 90min ,用原位杂交法和原位缺口末端标记 (TUNEL)法分别检测假手术组、对照组和亚低温组的谷氨酸载体EAAC1mRNA表达阳性率和凋亡细胞百分率。结果 与对照组比较 ,亚低温组和假手术组的EAAC1mRNA表达阳性率和凋亡细胞百分率均明显降低 (P <0 0 5和P <0 0 1) ;而亚低温组EAAC1mRNA表达阳性率显著升高 (P <0 0 5 ) ,凋亡细胞百分率明显下降 (P <0 0 5 )。结论 亚低温的抗脑缺血再灌注损伤后损伤神经细胞凋亡作用可能与其上调EAAC1mRNA表达。

Objective To explore the effects of mild hypothermia on the glutamate transproter EAAC1mRNA expression and the injuried nervous cell apoptosis after rat focal cerebral ischemia/reperfusion injury. Methods The middle cerebral arteries(MCA) of SD rats were occluded for 30 minutes, and reperfused for 90 minutes. Using DIG-labeled CRNA probe and TUNEL, the positive rate of the glutamate transporter EAAC1mRNA expression and the apoptotic cell rate were determined in the sham-operated group, the control group and the mild hypothermia group separatively. Results The positive rate of the EAAC1mRNA expression and the apoptotic cell rate apoptotic cell rate were significantly lower in the sham-operated group than those in the control group separatively (P<0 05,P<0 01). The positive rate of the EAAC1mRNA expression was obviously higher and the apoptotic cell rate was significantly lower in the mild hypothermia group than those in the control group (P<0 05). Conclusions That the mild hypothermia decreases the injuried nervous cell apoptosis may be related to the increased EAAC1mRNA expression, the induced synthesis of EAAC1 and the increased uptake of glutamate.