鸡矢藤颗粒剂干预大鼠肝纤维化模型OPN与TGF-β1水平表达的试验研究

Experimental study on the expression level of OPN and TGF-β1 in rat liver fibrosis model with tuber granule intervention

将30只大鼠随机分为正常对照组、肝纤维化模型非治疗组和肝纤维化模型治疗组。8周后采用Western blotting检测OPN蛋白表达,采用荧光实时定量法检测TGF-β1 mRNA的表达水平。结果显示,采用鸡矢藤颗粒剂治疗组(模型治疗组)的HA、LN、PCIII、CIV放射免疫数据与正常组相比有显著差异(P <0. 01),同时与模型非治疗组比有差异(P <0.05);模型非治疗组的HA、LN、PCIII、CIV放射免疫数据与治疗组相比有显著差异(P <0. 01);采用鸡矢藤治疗(模型治疗组)的OPN蛋白表达数值水平较正常组有差异(t=2. 236,P <0. 05),同时较模型非治疗组有显著差异(t=1. 024,P <0. 01);采用鸡矢藤治疗(模型治疗组)的TGF-β1 mRNA的表达数值水平较正常组有差异(t=2. 245,P <0. 05),同时较模型非治疗组有显著差异(t=1. 142,P <0. 01)。说明鸡矢藤可以抑制OPN蛋白表达,抑制TGF-β1 mRNA的表达,减缓肝纤维化的进程。研究表明OPN与TGF-β1调控的信号通路具有相关性,具体信号通路的调控有待进一步研究。

Methods The 30 rats were randomly divided into normal control group,the non-treatment group of liver fibrosis model and the treatment group of liver fibrosis model. Each group of rats are take the following operation： Western blotting test OPN protein expression after eitht weeks.The PCR detection of TGF-β1 mRNA expression level. Results This study showed that the treatment group of liver fibrosis model of HA,LN,PCIII,CIV radioimmunoassay were higher than the treatment group have significant differences（P 〈0. 01）,and the differences were statistically significant than the non-treatment group of liver fibrosis model at the same time（P 〈0. 05）. The non-treatment group of liver fibrosis model of HA（Hyaluronic acid）,LN（Laminin）,PCIII（Procollagen type III）,CIV radioimmunoassay showed significant differences compared to the treatment group of liver fibrosis model（P〈 0. 01）. Using the treatment group of liver fibrosis model of OPN protein expression level,differences were found inthe normal group（t = 2. 236,P 〈0. 05）,and the differences were statistically significant than the non-treatment group of liver fibrosis model at the same time（t = 1. 024,P 〈0. 01）. Using the treatment group of liver fibrosis model of TGF-β1 mRNA expression level of numerical difference compared with normal group（t = 2. 245,P〈 0. 05）,and compared with the non-treatment group of liver fibrosis model had significant difference（t =1. 024,P 〈0. 01）. Conclusions The P. scandens granule could inhibit the OPN protein expression,inhibition of TGF-β1 mRNA expression,slow down the progress of liver fibrosis. Through the experiments,it showed that OPN associated with TGF-β1 regulation of signaling pathway and the specific signaling pathways regulating needs further research.