怀山药酵素粉的制备及抗氧化作用

Preparation and antioxidant activity of chinese yam ferment powder

通过单因素和正交试验优化怀山药酵素液的发酵条件,利用喷雾干燥技术制备怀山药酵素粉,分析怀山药酵素原液喷雾干燥前后超氧化物歧化酶(superoxide dismutase,SOD)活性的变化,最后利用邻苯三酚自氧化法和H_2O_2/Fe体系反应法测定其对超氧阴离子和羟自由基的清除能力。结果表明,怀山药酵素液的最佳发酵工艺参数为糖浓度12%、接种量6%、发酵时间30 h、发酵温度42℃,该条件下怀山药酵素液SOD的活性为15. 35 U/mL。喷雾干燥后的怀山药酵素粉SOD活性没有显著的变化(P> 0. 05)。抗氧化活性试验结果显示,怀山药酵素粉复原液能够有效地清除超氧阴离子和羟自由基。

The fermentation condition of Chinese yam ferment liquid was optimized with single factor experiments and orthogonal experiment. The Chinese yam ferment powder was prepared using spray drying. The changes in superoxide dismutase（SOD） activity between Chinese yam ferment liquid and recovery ferment liquid were analyzed. The abilities to remove superoxide anions and hydroxyl radicals were measured using the methods of pyrogallol autoxidation and H2O2/Fe system. the optimum fermentation process parameters of Chinese yam ferment liquid were as follow： sugar concentration 12%,inoculum concentration 6%,fermentation time 30 h,fermentation temperature 42 ℃. Under the control of this condition,the SOD activity of the Chinese yam ferment liquid was 15. 35 U/mL. There was no significant change in the SOD activity between Chinese yam ferment liquid and powder after spray drying（P〉 0. 05）. The results of antioxidant activity showed that the superoxide anion and hydroxyl radical could be effectively eliminated by the recovery ferment liquid.