BRAF V600E突变与甲状腺乳头状癌临床病理相关性研究

Analysis of clinicopathological characteristics of papillary thyroid carcinoma and BRAF V600E gene mutation

目的:研究BRAF V600E突变与甲状腺乳头状癌(PTC)临床病理特征的相关性,探讨PTC组织BRAF V600E突变检测的应用价值。方法:采用qPCR方法对108例PTC患者及30例对照组织进行BRAF V600E突变检测,回顾PTC突变型组和野生型组患者的临床病理资料,使用SPSS 21.0软件进行统计学分析。结果:对照组未检测到BRAF V600E突变。108例PTC患者BRAF V600E突变率为72.20%。突变型组患者平均年龄大于野生型组,差异有统计学意义(P<0.05)。BRAF V600E突变与PTC组织亚型有关,与中央区淋巴结转移有关,差异有统计学意义(P<0.05)。与肿瘤直径、性别、多灶性、癌灶部位、伴随疾病、颈侧区淋巴结转移、被膜侵犯及TNM分期无关(P>0.05)。结论:BRAF V600E突变对PTC预后判断的价值尚待研究,相比于预后判断,对PTC的病理诊断价值更加显著。

Objective：To study the correlation between the BRAF V600E mutation and the clinicopathological features of papillary thyroid carcinoma（PTC）,and to explore the application value of BRAF V600E mutation in PTC.Method：The BRAF V600E gene mutation was detected in 108 PTC cases and 30 control cases by qPCR.The results were statistically analyzed by using SPSS 21.0 software.Result：The BRAF V600E mutation was absent in the control group.The BRAF V600E mutation rate was 72.20% in 108 PTC cases.Compared with patients with wild-type,the mean age of patients with the mutant BRAF V600E was significantly older（P0.05）.BRAF V600E mutations were associated with PTC tissue subtypes and central area lymph node metastasis（P0.05）.BRAF V600E mutations were not correlated with tumor diameter,patient gender,multifocality,tumor site,concomitant disease,lymph node metastasis in side region of neck,capsule invasion,and TNM staging（P0.05）.Conclusion：The value of BRAF V600E mutation in the prognosis of PTC remains to be studied.BRAF V600E mutation is of value for pathological diagnosis of PTC.