甘糖酯对高脂血症大鼠血脂及脂蛋白脂酶的调节作用

EFFECT OF PROPYLENE GLYCOL MANNATE SULFATE ON BLOOD LIPIDS AND LIPOPROTEIN LIPASE IN HYPERLIPIDEMIC RAT

目的 探讨甘糖酯调节血脂的分子机制。方法 以不同剂量甘糖酯 (37 8,75 6mg·kg-1 ·d-1 )给高脂血症大鼠ig给药 3周 ,禁食 1 2h后 ,测定大鼠血清总胆固醇 (TC)、甘油三酯 (TG)、低密度脂蛋白胆固醇 (LDL C)和高密度脂蛋白胆固醇 (HDL C)的浓度变化 ,用反转录聚合酶链式反应 (RT PCR)法检测组织中脂蛋白脂酶 (LPL)mRNA的表达。结果 甘糖酯有降低血清TC ,TG和LDL C ,升高HDL C的作用 ,同时提高LPLmRNA的表达 ,且呈剂量依赖性关系。甘糖酯对LPLmRNA转录的促进作用与其降低TC ,TG和LDL C的作用呈正比关系。结论 甘糖酯通过促进LPLmRNA转录而调节血脂水平 ,这可能是甘糖酯降血脂。

AIM To study the effect of propylene glycol mannate sulfate (PGMS) on blood lipids and lipoprotein lipase in hyperlipidemic rat, and its anti hyperlipidemic mechanism. METHODS PGMS was administered ig at different doses (37 8 mg·kg -1 ·d -1 and 75 6 mg·kg -1 ·d -1 ) to hyperlipidemic rats for three weeks and blo od serum was obtained after starved 12 h. Total cholesterol (TC), triglyceride ( TG), low density lipoprotein cholesterol (LDL C) and high density lipoprotein c holesterol (HDL C) were examined. The mRNA expression of lipoprotein lipase (LP L) in liver, spleen and artery was detected by reverse transcription polymerase chain reaction (RT PCR). RESULTS PGMS significantly decreased the levels of TC, TG and LDL C and increased that of HDL C in hyperlipidemic serum dose dependently. PGMS was shown to increase the level of LPL mRNA expression, which is related directly to the controlling e ffects of PGMS on blood lipids. CONCLUSION PGMS modulated blood lipids by promoting mRNA expression of LPL. This may be one important mechanism of PGMS to modulate blood lipids.