天然蒙脱石与细菌相互作用机理的研究

STUDY ON THE MECHANISM OF THE INTERACTION BETWEEN MONTMORILLONITE AND BACTERIUM

目的 通过研究一系列带电性不同的蒙脱石与细菌相互作用 ,探讨蒙脱石与细菌作用的机理。方法 抑菌实验 :在含不同浓度蒙脱石的混悬状的平板培养基上 ,接种浓度 1× 1 0 6·mL-1 大肠杆菌、金葡菌各一环 ,在培养箱中培养 2 4h ,观察细菌生长 ;细菌吸附实验 :在含细菌数约为 1× 1 0 7·mL-1 的人工肠液中 ,加入不同量的蒙脱石 ,置于培养箱中培养 ,计数菌落数。结果 天然蒙脱石无抑菌或杀菌作用 ,但交换上有抑菌作用的金属离子或基团后 ,即有抑菌或杀菌功能。蒙脱石对细菌的作用主要是吸附 ,“车厢式”结构的蒙脱石凝胶能将细菌固定到“车厢”中的独特吸附方式是治疗腹泻的药理基础 ,且吸附力强弱与其带电性有关。结论 蒙脱石对细菌无抑制或杀灭作用 ,只有吸附作用 ,大小与带电量呈负相关目的 通过研究一系列带电性不同的蒙脱石与细菌相互作用 ,探讨蒙脱石与细菌作用的机理。方法 抑菌实验 :在含不同浓度蒙脱石的混悬状的平板培养基上 ,接种浓度 1× 1 0 6·mL-1 大肠杆菌、金葡菌各一环 ,在培养箱中培养 2 4h ,观察细菌生长 ;细菌吸附实验 :在含细菌数约为 1× 1 0 7·mL-1 的人工肠液中 ,加入不同量的蒙脱石 ,置于培养箱中培养 ,计数菌落数。结果 天然蒙脱石无抑菌或杀菌作用 ,但交换上有抑菌作?

AIM To investigate the mechanism of the interaction between montmorillonite and bact eria by studying the reactions of different charges of montmorillonites with bac teria. METHODS Bacteriostatic test: one loop of E.coli and Staphylococcus aureus at the concentration of 1×10 6·mL -1 was incubated to the plate culture medium containing different concentrations of montmorillonite, and incubated 24 h to o bserve the growth of bacteria. Bacterial adsorptive test: different amounts of m ontmorillonite were added into the artificially simulated intestinal solution (c ontaining bacteria 1×10 7·mL -1 ). After the culture, the bacterial colon ies were counted. RESULTS The results showed that montmorillonite per se showed no bacteriostatic or b actericidal effect, but after exchange with metal ion and functional groups whic h inhibits bacteria, then it showed these activities. Adsorption was the main wa y between montmorillonite and bacteria. The special way of fixing bacteria into the “carriage” of montmorillonite gel which carry this structure was its pharm acological basis of curing diarrhea. The adsorption effect was related to layer charge density of the montmorillonites. CONCLUSION Montmorillonite showed adsorption ability of bacteria with minus related to its layer charge, but has no bacteriostatic and bactericidal effect.