电针对脊髓损伤模型大鼠自噬相关基因Beclin-1表达的调控作用

The regulation of eleetroaeupuncture on the autophagy related gene beclin-1 expression of rats with spinal cord injure

目的研究电针对脊髓损伤模型大鼠自噬相关基因Beclin-1表达的影响。方法将36只SD大鼠按随机数字表法分为假手术组、模型组和电针组，每组12只。假手术组仅行椎板切除术，不损伤脊髓；模型组和电针组采用脊髓打击器制备脊髓损伤模型。造模后电针组给予电针干预，假手术组、模型组行同等条件抓取。每日行脊髓损伤行为学评分，造模后3d处死取材。采用免疫组化法检测大鼠脊髓Beclin-1表达，采用WB、qPCR法检测大鼠脊髓Beclin-1蛋白及mRNA表达。结果与假手术组比较，造模后3d，电针组脊髓损伤行为学评分[（2．94±0．22）分比（2．03±0.18）分]升高（P〈0．05）；电针组大鼠脊髓Beclinl阳性表达平均光密度[（0．64±0.20）比（0．80±0．17）]、Beclin-1蛋白[（0．59±0．22）比（0．71±0．19）]、Beclin-1mRNA[（0．55±0．12）比（0．77±0．14）]表达均降低（P〈0．05）。结论电针可下调脊髓损伤模型大鼠Beclin-1蛋白和mRNA表达，抑制自噬水平，这可能是其促进脊髓损伤模型大鼠肢体运动功能恢复的机制之一。

Objective To study the effect of electroacupuncture on the expression of autophagy related gene Beclin-1 after spinal cord injury in rats. Methods A total of 36 SD rats were randomly divided into sham operation group （Sham）, model group （Model） and electroacupuncture group （EA）, 12 rats in each group. The sham group underwent laminectomy, without spinal cord injury; Model group and EA group were treated with NYU spinal cord preparation model of spinal cord injury blow system. After the operation, the EA group was treated with electroacupuncture. The other groups were treated with the same conditions, and the BBB limb movement function was scored every day, and 3 days after the operation were sacrificed. The expression of Beclin-1 was detected by immtmohistochemical method, and the expression of Beclin-1 protein was detected by Western blot, and the expression of Beclin-1 mRNA was detected by qPCR. Results On the 3 day after operation, the BBB scores （2.94±0.22 vs. 2.03 ±0.18） of EA group was significantly higher than those in Model group （P〈0.05）. Compared with the model group, the mean light density （0.64 a： 0.20 vs. 0.80±0.17）, the expression of Beclin-1 protein （0.59±0.22 vs.0.71±0.19） and the Beclin-1 mRNA （0.55±0.12 vs. 0.77±0.14） expression of Beclinl positive expression in the electroacupuncture group significantly decreased, and the difference was statistically significant （P〈0.05）. Conclusions The expression of autophagy related gene Beclin-1 increased in spinal cord after spinal cord injury. EA could down regulate the expression of Beclin-1 protein and mRNA and inhibit autophagy level, which may be one of the reasons for electroacupuncture to promote the recovery of limb motor function in rats with spinal cord injury.