D-101型大孔吸附树脂分离纯化细叶鼠曲草总黄酮

Study on the techniques of purification flavones from Gnaphalium japonicum Thunb. by D-101 macroporous adsorption resin

目的研究D-101型大孔吸附树脂分离纯化细叶鼠曲草总黄酮的最佳工艺条件，并测定细叶鼠曲草不同部位总黄酮的含量。方法以细叶鼠曲草总黄酮的吸附率、解吸率为指标，考察D-1叭型大孔吸附树脂对细叶鼠曲草中总黄酮的纯化能力，采用静态吸附法和动态吸附法分析静态饱和吸附量、静态洗脱率、上样量浓度、上样液pH、洗脱液浓度和洗脱液用量对吸附解吸的影响；以芦丁为对照品，测定总黄酮含量。结果D．101型大孔吸附树脂对细叶鼠曲草总黄酮分离纯化效果良好，最佳吸附和解吸条件为：上样量浓度为1．0mg，ml、上样液pH=4、解吸液乙醇浓度为60％、解吸流速为2．5BVm，在此条件下细叶鼠曲草总黄酮纯度为60．92％；细叶鼠曲草不同部位黄酮含量为：茎叶〉花〉根部。结论在最佳纯化分离条件下，D-1ol型大孔吸附树脂分离纯化细叶鼠曲草总黄酮纯度提高；细叶鼠曲草茎叶中黄酮含量最高。

Objective To investigate the optimal isolation and purification conditions offlavonoids in Gnaphlium affine Thunb. and the content of flavonoids in different parts of Gnaphlium affine Thunb. was measured. Methods The separation and purification abilities of D-101 macroporoue adsorbing resins for flavonoids in Gnaphliurn affine Thunb. were studied with adorption and desorption as index. The static adsorption and dynamic adsorption methods were used to analyze the effects of static saturated adsorption, static elution rate, sample concentration, sample pH value, eluent concentration and amount of eluent. The flavonoids concentrations were determined with rutin as standard. Results The D-101 macroporous adsorption resin had good effect on the separation and purification of flavonoids from Gnaphlium affine Thunb.; The optimal conditions for purification were： sample concentration 1.0 mg/ml, sample pH=4, 60% ethanol as desorption solvent, washing flow 2 BV/h, with these parameters. With such condition, the purity of flavonoids in Gnaphliurn affine Thunb. was 60.92%; The content of fiavonoids in Gnaphlium affine Thunb. showed the highest content was in the stem, the second in the flower, and the least in the root. Conclusions The purification of flavonoids from the D-101 macroporous adsorption resin increased in the best purified separation conditions and the content of flavonoids was the highest in the leaf.