桃ERF转录因子家族生物信息学分析及芽萌发相关基因筛选

Genome-wide Identification of Ethylene Responsive Factor(ERF) Family Genes in Peach and Screening of Genes Related to Germination

以中油四号油桃(Prunus persica var.nectarina)为研究对象,利用MEGA 6.0、MEME、GSDS和DNAMAN 6.0等软件对桃ERF家族数据进行生物信息学分析,鉴定得到102个ERF转录因子家族基因,并通过构建系统进化树将这102个基因分为10个子家族(I–X)。基因结构分析表明,有81个基因不含内含子,20个基因含有1个内含子,有1个基因与其它成员差异较大,含有5个内含子。保守元件分析表明,ERF家族包含20个保守元件,其中Motif 1、Motif 2和Motif 4都属于AP2/ERF结构域,同一个保守元件主要出现在同一个子家族中,并且大部分保守元件的功能未知。VIII子家族基因的荧光定量PCR分析表明,在桃叶芽处于不同的发育状态时,Ppe ERF068的表达量存在较大差异,光照培养箱中培养的桃芽在萌发过程中各时期表达量变化趋势进一步表明该基因可能与叶芽萌发有关,将其命名为Ppe EBB1。该研究为进一步揭示Ppe EBB1的分子机制奠定了基础,并为桃树的栽培管理和熟期调控了提供理论指导。

In this study, genome-wide identification of ERF family genes in ＇zhongyousihao＇ peach was studied by using MEGA 6.0, MEME, GSDS, DNAMAN 6.0. We identified 102 genes, and phylogenetic analyses of the genes indicated that the transcription factors can be classified into 10 different groups （I-X）. Gene structure analysis showed that 81 genes had no introns, 20 genes contained an intron, and 1 gene contained 5 introns, which had a large difference from other members. Conservative motif analysis showed that the ERF family contained 20 conserved elements： Motifs 1, 2 and 4 belonged to the APETALA2 （AP2）/ERF domain, and the same conserved motif appeared mainly in the same family; the function of most conserved motifs is unknown, qRT-PCR revealed that the expression of PpeERF068 during dormancy and germination was consistent with the developmental status of leaf buds, which suggested that it might be related to leaf bud germination. The gene expression of peach buds during each period in light culture incubation further showed the relation between PpeERF068 and the germination of leaf buds. So PpeERF068 was named PpeEBBI. We will further study the molecular mechanism of PpeEBB1 to provide theoretical guidance for the cultivation and management of peach.