水稻雄配子体发育过程中tRNA片段的生物信息学分析

Bioinformatics Analysis of tRNA-derived Fragments in Rice Male Gametophyte Development

tRNA片段(t RF)是t RNA通过非随机剪切产生的RNA片段,其产生和功能机制尚不明确;而在水稻(Oryza sativa)雄配子体发育过程中,人们对t RNA更是知之甚少。通过高通量测序,在水稻雄配子体发育过程中发现了长度范围较大的t RFs;进一步采用logo对t RFs两端的序列进行分析,发现了4个有序列特征(其中3个未见报道)和1个无序列特征的酶切位点;通过NCBI Blast预测了t RF靶基因,发现其大多靶向转座因子。研究结果对揭示t RF产生机制以及水稻雄配子体发育研究有一定的参考价值。

tRNA derived fragments （termed tRFs） are functional RNAs produced by non-random cleavage of tRNAs. The knowledge of tRNAs＇ production and function is being studied but is unknown in the rice male gametophyte. Using high-throughput sequencing with 3 stages in the rice male gametophyte development, we found tRFs with a large range of length. Next, we determined four sequence-specific and one non-sequence-specific ribonuclease cleaving sites. We also summarized the distribution profile of tRFs in the three stages. Finally, a NCBI blast search was carried out to predict the tRF target gene, revealing that the major class of targets was transposable elements. This results provid new clues for rice male gametophyte research.