一种简单有效的非损伤观测根瘤和根系形态的方法

An Observation Method of Nodule and Root Morphology without Damage in Real-time

实时观测根瘤及根系形态对于豆科植物研究具有重要意义,但目前还缺乏一个便于观测根系、高效结瘤、适宜生长且经济实用的豆科植物培养体系。以蒺藜苜蓿(Medicago truncatula)为植物材料,建立了一种可实时观测根瘤及根系形态的纸袋水培法,并与其它常用方法进行对比。结果表明,依赖于石英砂等固体介质栽培蒺藜苜蓿对根瘤和根系形态的实时观测造成障碍,而水培和喷雾培养等方法的根瘤菌接种效率不高,且不便观测侧根发育情况。采用纸袋水培法探讨了褪黑素对蒺藜苜蓿根系发育的影响,发现褪黑素具有降低根瘤形成效率、抑制侧根伸长、增加侧根数目以及增大侧根与主根之间夹角等作用。因此,纸袋水培法能够高效接种根瘤菌且为实时无损伤观测根瘤及根系形态提供了可能,是一种适用于豆科植物简单有效的培养方法。

Observation of root and nodule morphology in real-time has great significance in the study of Leguminosae. However, we lack a legume plant culture system that is convenient for observing root morphology and with efficient Rhizobium inoculation and also suitable for plant growth and economical. In this work, we compared the efficacy of a paper-based Ziplock bag hydroponics method with other commonly used methods for real-time observation of nodules and root morphology of Medicago truncatula. Methods relied on quartz sand and other solid medium cultivation. M. trun- catula had obstacles to observe the nodule characteristics and root morphology in real-time. Rhizobium inoculation was also inefficient with hydroponics and aeroponics, and the methods were not suitable for observing lateral root develop- ment. Moreover, we also studied the effects of melatonin on the development of root morphology of M. truncatula with the paper-based Ziplock bag hydroponics method. Although melatonin reduced the number of nodules and inhibited the lat- eral root elongation, the number of lateral roots and the angle of the lateral root with the main root increased. The pa- per-based Ziplock bag hydroponics method is a simple and effective culture method for leguminous plants, with efficient Rhizobium inoculation, which allows for observing root nodules and root morphology in real-time with non-damaged roots.