Fascin和survivin蛋白在结直肠癌组织中的表达及临床意义

Expression and significance of Fascin and survivin protein in colorectal cancer tissues

目的探讨肌成束蛋白(Fascin)和存活蛋白(survivin)蛋白在结直肠癌组织中的表达及意义。方法收集67例结直肠癌患者的结直肠癌组织标本及其癌旁组织标本,采用免疫组织化学染色法检测Fascin和survivin蛋白的表达水平,分析Fascin和survivin蛋白表达情况与结直肠癌患者临床特征的关系。采用Logistic回归分析法分析影响结直肠癌患者Fascin和survivin蛋白表达的因素。结果结直肠癌组织中survivin和Fascin蛋白的阳性表达率分别为65.67%和52.24%,明显高于癌旁组织的13.43%和0,差异均有统计学意义(P﹤0.01)。Dukes分期为C～D期、有淋巴结转移的结直肠癌患者的survivin蛋白阳性表达率明显高于Dukes分期为A～B期、无淋巴结转移的患者(P﹤0.01);Dukes分期为C～D期、有淋巴结转移、中低分化的结直肠癌患者的Fascin蛋白阳性表达率明显高于Dukes分期为A～B期、无淋巴结转移和高分化患者(P﹤0.01)。survivin与Fascin蛋白的表达呈正相关(rs=0.441,P﹤0.05);Logistic回归分析结果显示,Dukes分期和淋巴结转移情况是survivin蛋白阳性表达的独立影响因素(OR=3.508、3.016,P﹤0.01),Dukes分期、淋巴结转移情况和分化程度是Fascin蛋白阳性表达的独立影响因素(OR=3.022、2.787、2.627,P﹤0.01)。结论结直肠癌组织中Fascin和survivin蛋白呈高表达,且其阳性表达与患者的临床特征有关,可能在结直肠癌的发生发展中发挥一定的作用,两者可能具有协同作用。

Objective To investigate the expression and significance of Fascin and survivin protein in colorectal cancer tissues. Method 67 cases of colorectal cancer patients were included in the study, from which the colorectal cancer tissues and adjacent normal tissues were collected and detected by immunohistochemical staining to determine the expression of Fascin and survivin proteins, and then the correlation between protein expression and clinical characteristics of colorectal cancer patients were investigated. Logistic regression analysis was used to analyze the factors influencing the expression of Fascin and survivin proteins. Result The positive expression rates of survivin and Fascin protein in colorectal cancer tissues were 65.67% and 52.24%, which were significantly higher than those in adjacent tissues at13.43% and 0, respectively, demonstrating statistically significant difference（P〈0.01）. The positive expression rate of survivin protein in patients with Dukes stage C-D disease and lymph node metastases were significantly higher than those in patients with Dukes stage A-B disease and no lymph node metastases（P〈0.01）; while for Fascin protein, significantly higher positive expression rate was observed in patients with Dukes stage C-D disease, lymph node metastases and moderate or poor differentiation compared with that in patients with Dukes stage A-B disease, no lymph node metastases and high differentiation（P〈0.01）. The expression of survivin protein was positively correlated with the expression of Fascin protein（rs=0.441, P〈0.05）; Logistic regression analysis showed that Dukes staging and lymph node metastases were independent risk factors for positive survivin expression（OR=3.508, 3.016, P〈0.01）, and Dukes staging, lymph node metastasis and differentiation were associated with the positive expression of Fascin protein（OR=3.022, 2.787, 2.627, P〈0.01）.Conclusion Fascin and survivin protein in colorectal cancer tissues are highly expressed, which are related to the clinical characteristics of patients, and they may be involved in the occurrence and development of colorectal cancer, showing a synergistic effect.