瓦苇属蓝镜玉露的离体培养与快速繁殖

In Vitro Culture and Rapid Propagation of Blue Mirror Jade Dew(Haworthia)

[目的]研究蓝镜玉露的离体培养与快速繁殖。[方法]蓝镜玉露花蕾接种于不同培养基后,研究不同生长调节剂及其配比对其愈伤组织诱导、分化、增殖及植株再生的影响。[结果]适宜的外植体培养基为MS+6-BA 1.0 mg/L+TDZ 0.3 mg/L+蔗糖30 g/L+琼脂7 g/L,外植体脱分化速度快,愈伤组织质量好;适宜的分化培养基为MS+6-BA 1.0 mg/L+TDZ 0.5 mg/L+蔗糖30 g/L+琼脂7 g/L;适宜的增殖培养基为MS+6-BA 0.4 mg/L+TDZ 0.3 mg/L+蔗糖30 g/L+琼脂7 g/L,增殖系数达5.0。对增殖后的组培苗无需生根处理直接炼苗,炼苗生根成活率高达95%以上。[结论]通过蓝镜玉露的花蕾再生及组培快繁可为工厂化生产提供理论依据。

[ Objective ] To study the in vitro culture and rapid propagation of Blue Mirror Jade Dew. [ Method ] After the flower buds of Blue Mirror Jade Dew was inoculated onto different culture mediums, we researched the effects of different growth regulators and their mixtures on callus induction, differentiation, proliferation and plant regeneration. [ Result] The suitable explant culture medium was MS＋6-BA 1.0 mg/L ＋TDZ 0.3 mg/L＋sucrose 30 g/L＋agar 7 g/L, and the explants dedifferentiated quickly and the callus quality was good under these conditions. The suitable differentiation medium was MS＋6- BA 1 mg/L＋TDZ 0.5 mg/L＋suerose 30 g/L＋agar 7 g/L. The suitable proliteration medium was MS＋6-BA 0.4 mg/L＋TDZ 0.3 mg/L＋suerose 30 g/L＋agar 7 g/L, and the proliteration coefficient was 5.0 under these conditions.After the propagation of the tissue culture seedlings, no rooting treatment was needed, and the survival rate of the seedlings was as high as 95%. [ Conclusion] The study of flower bud regeneration and tissue rapid propagation of Blue Mirror Jade Dew can provide a theoretical basis for large-scale production.