槐米及其制品中总黄酮含量测定方法的研究

Study on Determination Method of Total Flavones in Sophora Japonica and Its Products

[目的]建立一种科学、准确的同时适用于槐米、槐米提取液以及槐米发酵液等槐米制品中总黄酮测定方法。[方法]采用紫外分光光度法,通过紫外扫描确定最大吸收波长,并对溶剂浓度、还原剂用量、还原反应时间、络合剂用量等参数进行条件优化,以使黄酮准确地被检测出来,避免假阳性干扰;以芦丁为对照品制定标准曲线,并进行样品溶液的精密度实验、回收率实验及实验室间验证实验。[结果]最大吸收波长为510nm,在10mg/L～40mg/L范围内呈现良好的线性关系,相关系数为0.99578。精密度、重现性及回收率满足准确性要求。[结论]该方法线性关系良好,精密度高,重现性良好,可作为槐米及其制品中黄酮类化合物快速测定的方法,为槐米提取、纯化工艺及槐米制品的制备等相关研究提供了一种客观、科学、准确的定量方法。

[Objective] To establish a scientific accurate determination method of total flavones in Sophora japonica and its products such as sophora japonica extract and fermentation broth. [Method] The UV spectrophotometric method is used,through the ultraviolet scan determination Max absorption wavelength. We get tuning parameters by making the contrast of solvent strength,dosage of reducing agent,reaction time,mass ofcomplexant to measure accurately and avoid false positive interference. Then we take the Rutin as the comparison formulation specification curve and carry on the accuracy experiment,recovery experiment and labs＇ validation tests.[Result]The Max absorption wavelength is 510 nm and the specification curve linearity range is 10-40 mg/L. The linearly dependent coefficient is 0.99578 and the precision,reproducibility,recovery rate can satisfy the request.[Conclusion] This method has good linear relation, good degree of accuracy and precision, which is a rapid and accurate determination method for sophora japonica and its products and provides the basis forSophora japonica＇s extract, purification and preparation of Sophora japonica＇s products.