紫花玉簪内生真菌分离鉴定及其抗氧化活性研究（英文）

Isolation, characterization and antioxidant activities of the endophytic fungi from Hosta. Ventricosa

以紫花玉簪为研究对象,从其根部分离得到13株内生真菌,并通过IST序列扩增后18S rDNA进行鉴定。以总抗氧化能力,DPPH自由基清除能力,ABTS+在自由基清除能力为指标,研究13种内生真菌菌丝体醇提物抗氧化活性,并测定其总多酚含量。结果表明,HoV1 (尖孢镰刀菌属)和Ho V4 (附球菌属)粗提物多酚含量较高(1.90±0.03 mg/g,2.41±0.01 mg/g),且表现出较强的综合抗氧化能力。其总抗氧化能力分别为(63.46±0.26 U/g,66.05±0.71 U/g),ABTS^+自由基清除IC_(50)分别为(30.25±0.05μg/mL,24.66±0.65μg/mL),DPPH自由基清除IC_(50)分别为(83.42±0.24μg/mL,59.136μg/mL)。另外,尖孢镰刀菌属的HoV8具有优于BHT的DPPH自由基清除活性(IC_(50)=16.02μg/mL)。这些真菌都有望开发成为新型天然抗氧化制剂。

A total of 13 types of endophytic fungi,classified into six genera,were isolated from Hosta.ventricosa based on 18 S rDNA sequencing of ITS region and microscopic examination.The antioxidant activities of the crude extracts were investigated by T-AOC,DPPH and ABTS＋ methods.The results showed that the crude extracts from Fusarium oxysporum（HoV1） and Epicoccum（HoV4）,which presented higher phenol levels（1.90±0.03 mg/g,and 2.41±0.01 mg/g,respectively）,exhibited stronger comprehensive antioxidant activities,with higher T-AOC values（63.46±0.26 U/g,and 66.05±0.71 U/g,respectively）,lower EC_（50） values against ABTS~＋（30.25±0.05 μg/m L,and 24.66±0.65 μg/m L,respectively） and DPPH radicals（83.42±0.24 μg/m L,and 59.136 μg/m L,respectively）.Moreover,Fusarium oxysporum（Ho V8） showed excellent scavenging activity against DPPH radicals（EC_（50） = 16.02 μg/m L）,which was better than BHT.All of them could be useful in the developing of new natural antioxidant agents.