降香提取物B3对斑马鱼模型促血管新生作用的研究

Study on the extract B3 of Dalbergia on angiogenesis of zebrafish model

目的:评价降香提取物B3的促新血管生成作用及其机理。方法:采用转基因斑马鱼模型,通过观察转基因斑马鱼肠下血管(SIVs)新生模型及节间血管(ISVs)损伤模型来评价降香提取物B3的促新血管生成作用及其机理。结果:降香提取物B3能剂量依赖性地促进转基因斑马鱼SIVs区域的毛细血管出芽新生,30μg/ml效果最佳;能修复VEGF受体激酶抑制剂Ⅱ(VRI)诱导的ISVs区域血管损伤,且能上调VEGF受体激酶抑制剂Ⅱ诱导后的kdr、kdrl及flt-1等VEGF受体mRNA水平的下降。结论:降香提取物B3能通过调控VEGF受体mRNA水平以发挥其在转基因斑马鱼上的促血管新生效果,具有被开发为治疗性血管新生药物的潜力。

Objective： To evaluate the Dalbergia extract B3 promoting angiogenesis and its mechanism. Methods： In the transgenic zebrafish,neonatal sub intestinal veins（SIVs） model and intersegmental vessels（ISVs） injury model were used to evaluate Dalbergia extract B3 promoting angiogenesis and its mechanism. Results： Extract B3 of Dalbergia dose-dependently promoted capillaries to sprout in the transgenic zebrafish SIVs region and 30 g/ml was the optimized dose. Extract B3 of Dalbergia repaired VEGF receptor kinase inhibitor（VRI） induced vascular damage in the ISVs region,and upregulated decreased levels of kdr,kdrl and Flt-1 mRNA. Conclusion： Extract B3 of Dalbergia could regulate VEGF receptor mRNA level in transgenic zebrafish to exert its angiogenic effect.