摘要
目的:评价降香提取物B3的促新血管生成作用及其机理。方法:采用转基因斑马鱼模型,通过观察转基因斑马鱼肠下血管(SIVs)新生模型及节间血管(ISVs)损伤模型来评价降香提取物B3的促新血管生成作用及其机理。结果:降香提取物B3能剂量依赖性地促进转基因斑马鱼SIVs区域的毛细血管出芽新生,30μg/ml效果最佳;能修复VEGF受体激酶抑制剂Ⅱ(VRI)诱导的ISVs区域血管损伤,且能上调VEGF受体激酶抑制剂Ⅱ诱导后的kdr、kdrl及flt-1等VEGF受体mRNA水平的下降。结论:降香提取物B3能通过调控VEGF受体mRNA水平以发挥其在转基因斑马鱼上的促血管新生效果,具有被开发为治疗性血管新生药物的潜力。
Objective: To evaluate the Dalbergia extract B3 promoting angiogenesis and its mechanism. Methods: In the transgenic zebrafish,neonatal sub intestinal veins(SIVs) model and intersegmental vessels(ISVs) injury model were used to evaluate Dalbergia extract B3 promoting angiogenesis and its mechanism. Results: Extract B3 of Dalbergia dose-dependently promoted capillaries to sprout in the transgenic zebrafish SIVs region and 30 g/ml was the optimized dose. Extract B3 of Dalbergia repaired VEGF receptor kinase inhibitor(VRI) induced vascular damage in the ISVs region,and upregulated decreased levels of kdr,kdrl and Flt-1 mRNA. Conclusion: Extract B3 of Dalbergia could regulate VEGF receptor mRNA level in transgenic zebrafish to exert its angiogenic effect.
作者
范竹鸣
王佑华
周昕
杨爱玲
林赟霄
樊华
李瑶
彭珑萍
刘宗军
王大英
Fan Zhumin;Wang Youhua;Zhou Xin;Yang Ailing;Lin Yunxiao;Fan Hua;Li Yao;Peng Longping;Liu Zongjun;Wang Dajun(Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062;Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2018年第4期66-69,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金(No.81403236,No.81328025)
上海市自然科学基金(No.12ZR1432200)
上海市科研计划项目(17401901600,15401902500)