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锁阳乙酸乙酯提取物对Aβ_(25-35)诱导损伤PC12细胞的影响 被引量:11

The effect of Cynomorium ethyl acetate extract containing serum on the Injured PC12 Cells Induced by Aβ_(25-35)
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摘要 目的:探讨锁阳乙酸乙酯提取物对Aβ_(25-35)诱导PC12细胞损伤的影响。方法:将48只大鼠随机分为空白组、锁阳乙酸乙酯提取物83mg/kg、8. 3mg/kg、0. 83mg/kg剂量组,采血制备空白血清和含药血清;以Aβ_(25-35)诱导PC12细胞损伤造模,锁阳乙酸乙酯提取物含药血清处理PC12细胞后,通过MTT法检测细胞活力,Hoechst 33342染色、流式细胞术检测PC12细胞凋亡情况,用相应的试剂盒测定细胞中超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮合酶(NOS)和一氧化氮(NO)的活性及含量。结果:与空白血清组相比,锁阳乙酸乙酯提取物各组10%血清显著提高细胞存活率,使细胞凋亡率明显下降,显著提高了SOD活性,显著降低了MDA、NOS、NO含量。结论:锁阳乙酸乙酯提取物对Aβ_(25-35)致PC12细胞损伤具有保护作用,其保护机制与其增强SOD活性,降低MDA含量,降低NOS活性,减少NO生成,减轻氧化应激对细胞的损伤有关。 Objective: To investigate the Cynomorium ethyl acetate extract(The ethyl acetate extract of Cynomorium songaricum,ECS) of A beta25-35 induced PC12 cell injury. Methods: 48 rats were randomly divided into control group,ECS high dose group(83 mg/kg),ECS medium dose group(8. 3 mg/kg) and ECS low dose group(0. 83 mg/kg). Preparation of blank sera and serum containing drugs by blood sampling; The PC12 cells were induced by Aβ25-35 and PC12 cells were treated with ECS containing serum. The cells viability detectioned by MTT. The apoptosis of PC12 cells was detected by Hoechst 33342 staining and flow cytometry. The activity and content of SOD,MDA,NOS and NO in the cells were measured with the corresponding kit. Results: Compared with the blank serum group,(1)the Cynanchum paniculatum extract group significantly increased the cell viability(P 〈 0. 01);(2)the cell apoptosis rate decreased significantly(P 〈 0. 01);(3)significantly increased the activity of SOD(P 〈 0. 01,P 〈 0. 05) and reduced MDA content(P 〈 0. 01,P 〈 0. 05),NOS activity(P 〈 0. 05) and NO content(P〈 0. 01). Conclusion: Cynanchum paniculatum ethyl acetate extract can protect PC12 cells induced by Aβ25-35,and its protective mechanism is related to the enhancement of SOD activity,the decrease of MDA content,the decrease of NOS activity,the decrease of NO production,and the reduction of oxidative stress on cell injury.
作者 曹俊彦 韩瑞兰 郭俊英 张亚男 Cao Junyan;Han Ruilan;Guo Junying;Zhang Yanan(Inner Mongolia Medical University,Huhhot,Inner Mongolia 010110)
机构地区 内蒙古医科大学
出处 《中药药理与临床》 CAS CSCD 北大核心 2018年第4期88-91,共4页 Pharmacology and Clinics of Chinese Materia Medica
基金 内蒙古自治区自然科学基金项目(No.2013MS1223)
关键词 锁阳乙酸乙酯提取物 Β-淀粉样蛋白 PC12细胞 氧化应激 Cynomorium ethyl acetate extract amyloid beta protein PC12 cells oxidative stress
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