WISP-1,β-catenin,L1CAM在食管鳞状细胞癌中的表达及意义

Expression and clinical significance of WISP-1,β-catenin and L1CAM in esophageal squamous cell carcinoma

目的:分析Wnt诱导的分泌型蛋白1(Wnt-induced secreted protein-1,WISP-1),β-catenin,L1CAM在食管癌组织中的表达及相关性,及三者与临床病理特征的关系。方法:采用免疫组化En vision两步法检测60例食管癌组织和57例癌旁组织WISP-1,β-catenin,L1CAM蛋白的表达。结果:(1) 60例食管鳞状细胞癌中WISP-1、β-catenin、L1CAM的阳性表达率或异常表达率,分别为66. 7%,100%和43. 3%,均高于癌旁组织的阳性表达率(9. 6%、0和3. 6%),差异均具有统计学意义(P <0. 001)。(2) WISP-1的表达与肿瘤分化程度有关(P=0. 01),高分化鳞癌表达比率和强度高于低分化癌,而与性别、浸润深度、肿瘤分期、淋巴结转移无关。β-catenin表达与肿瘤分期相关(P=0. 034),分期越高,表达缺失越多,与性别、浸润深度、分化程度、淋巴结转移不相关。L1CAM的表达与肿瘤分化程度、浸润深度、淋巴结转移、肿瘤分期有关(P <0. 05),与性别无明显相关性。(3)食管鳞癌中WISP-1与L1CAM之间的表达及WISP-1与β-catenin异常表达之间无相关性。L1CAM与β-catenin异常表达有显著相关性,β-catenin肿瘤细胞胞质和核阳性者,前者的阳性细胞数和比例增高(r=-0. 495,P <0. 001)。结论:WISP-1、L1CAM蛋白在食管癌表达的增加,以及β-catenin异常表达均与食管癌的发生发展密切相关; L1CAM表达与β-catenin核表达在食管癌的侵袭、转移中发挥重要作用。联合检测WISP-1,β-catenin,L1CAM有望成为食管癌评估预后的生物学指标,为食管癌治疗提供新靶点。

Objective： To investigate the expression of Wnt-induced secreted protein-1 （WISP-1）,β-catenin and L1CAM in esophageal squamous cell carcinoma and to analyze their clinicopathologic significance,and correlation of the three protein expression with clinicopathological features was determined. Methods： Expression of WISP-1,β-catenin and L1CAM in 60 ESCC specimens and 57 cases of normal esophageal mocusa tissues was detected by immunohistochemistry （IHC）. Results： The WISP-1,β-catenin and L1CAM positive/abnormal expression rate of 66.7%,100% and 43.3%,respectively,which were significantly higher than that of 9.6%,0 and 3.6% in control group（ P 〈0.001）.The expression of WISP-1 was correlated with the degree of tumor differentiation （ P =0.01）.The expression rate and intensity of WISP-1 in well-differentiated squamous cell carcinoma were higher than those in poorly differentiated carcinoma,but not with sex,depth of invasion,tumor stage and lymph node metastasis.The low expression of β-catenin was significantly correlated with tumor stage （ P =0.034）.The higher the stage,the more the loss of expression was,which was not related to sex,depth of invasion,degree of differentiation and lymph node metastasis.L1CAM expression was correlated with tumor differentiation,invasion depth,lymph node metastasis and tumor stage （ P 〈0.05）,but not with sex.There was no correlation between the expression of WISP-1 and L1CAM in esophageal squamous cell carcinoma and the abnormal expression of WISP-1 and β-catenin.There was a significant correlation between L1CAM and abnormal expression of β-catenin.The number and proportion of positive cells in cytoplasm and nucleus of β-catenin tumor cells were increased （ r=-0.495,P 〈0.001）. Conclusion： The over-expression of WISP-1 and L1CAM,as well as aberrant levels of β-catenin expression,may play an important role in the progression of ESCC.L1CAM expression and β-catenin expression play an important role in invasion and metastasis of esophageal cancer.The combined detection of WISP-1,β-catenin and L1CAM is expected to be a biological marker for evaluating the prognosis of esophageal cancer and provide a new target for the treatment of esophageal cancer.