当归补血汤对糖尿病大鼠肝脏、骨骼肌中PKC-ζ表达的影响

Effect of Danggui Buxue decoction on the expression of PKC-ζ in the liver and skeletal muscle of diabetic rats

[目的]研究当归补血汤对糖尿病大鼠肝脏、骨骼肌中蛋白激酶C-ζ(PKC-ζ)表达的影响。[方法]选取雄性SD大鼠50只,随机分为空白对照组、当归补血汤对照组、2型糖尿病(T2DM)模型组、T2DM+当归补血汤组、2型糖尿病+吡格列酮组。采用链脲佐菌素(STZ)联合高脂高糖饲料建立大鼠糖尿病模型,造模成功后,药物干预5周,取血检测大鼠空腹血糖(FPG)、空腹胰岛素(FINS)水平。用免疫组织化学法检测各组大鼠肝脏、骨骼肌细胞中PKC-ζ表达。[结果]与空白对照组比,T2DM模型组FPG、FINS显著升高;与T2DM模型组相比,T2DM+当归补血汤组和T2DM+吡格列酮组FPG、FINS下降。通过免疫组化检测发现,与空白对照组比,T2DM模型组肝脏和骨骼肌中PKC-ζ的表达下降;T2DM+当归补血汤组和T2DM+吡格列酮组较T2DM组表达升高,且T2DM+当归补血汤组与T2DM+吡格列酮组组间无明显差异。[结论]当归补血汤能够改善胰岛素抵抗、降低血糖,这可能与其促进肝脏、骨骼肌中PKC-ζ的表达有关。

[Objective] To explore the effect of Danggui Buxue decoction on the protein kinase C-ζ expression in the liver and skeletal muscle cell of diabetic rats. [Methods] Fifty male SD rats were randomly divided into normal control group （NC group）, Danggui Buxue decoction control group （DG group）, type 2 diabetes mellitus group （T2DM group）, type 2 diabetes mellitus ＋ Danggui Buxue Decoction group （T2DM ＋ DG group）, Upe 2 diabetes ＋ pioglitazone group （T2DM ＋ BG group）. Type 2 diabetes mellitus rat models were established by high fat diets and intraperitoneal injection with sreptozotocin （STZ）. After 5 weeks, measuring fasting blood glucose （FPG） and fasting insulin （FINS） in blood and using immunohistochenlical method to detect the content of PKC-ζ in the liver, skeletal muscle in rats seach group. [Results] Compared with NC group, the FPC and FINS of T2DM group increased significantly. Compared with T2DM group, the FPC and FINS of T2DM＋DC group and T2DM＋BC group decreased. The expression of PKC-ζ in the liver and skeletal muscle cell membrane of T2DM group was decreased by immunohistochemistry, while the expression of PKC-ζ in T2DM＋DG group and T2DM ＋ BG group was higher than that of T2DM group. Except that, there was no significant difference between T2DM＋DC group and T2DM ＋BC group. [Conclusion] Danggui Buxue decoction can improve insulin resistance and reduce blood sugar, which may be related to promoting the expression of PKC-ζ in the liver and skeletal muscle cells.