HPLC法测定保健食品中阿斯巴甜的含量

Simultaneous Determination of Aspartame in Health Food

目的建立HPLC法测定保健食品中阿斯巴甜的含量。方法采用Thermo Acclaim^(TM) 120 C_(18)色谱柱(250mm×4. 6mm,5μm),流动相为甲醇-水(39∶61);流速0. 8mL·min^(-1),进样量为10μL,检测波长208nm,柱温25℃。结果阿斯巴甜在2. 566~64. 152μg·mL^(-1)范围内线性关系良好(r=0. 9994),回收率为97. 4%,RSD 1. 9%(n=9)。结论本法简便、可靠、准确,可用于保健食品中阿斯巴甜含量的测定。

OBJECTIVE To establish an HPLC method for determination of aspartame in health food. METHODS The method was carried out on a Thermo Acclaim TM 120 C 18 column （250mm×4.6mm, 5μm）. The mobilephase was methol-water （39∶ 61）.The flow rate was 0.8mL·min -1,the samplesize was 10μL,the detective wavelength was 208nm and the column temperature was 25℃. RESULTS Aspartame have a good linear correlation within the range of 2.566～64.152μg·mL -1 （r=0.9994）.The average recoverywas 97.4%,RSD was 1.9%（n=9）. CONCLUSION The method is simple,reliable and accurate for determination of aspartame in health food.