高效液相色谱法测定蛋黄油软膏中维生素E的含量

Determination of Vitamin E in Egg Yolk Oil Ointment By HPLC

目的建立高效液相色谱法同时测定复方蛋黄油软膏中维生素E(γ-生育酚、α-生育酚)的含量。方法采用高效液相色谱法,色谱柱为SunFire C_(18)(250×4. 6mm,5μm),流动相为乙腈-水(96∶4),流速为1. 0mL·min^(-1),检测波长为292nm,进样体积为20μL,色谱柱柱温为30℃。结果γ-生育酚、α-生育酚的线性范围分别为5. 01~50. 20μg·mL^(-1)、50. 82~508. 75μg·mL^(-1)、,平均回收率分别为98. 20%、99. 20%,RSD分别为1. 3%、2. 0%。三批样品中γ-生育酚、α-生育酚的含量范围为0. 0231~0. 0234mg·g^(-1),0. 2586~0. 2867mg·g^(-1)。结论本方法简单、快速,可用于蛋黄油软膏中维生素E(γ-生育酚、α-生育酚)的含量测定。

OBJECTIVE To establish a method for determination of Vitamin E（γ-tocopherol and α-tocopherol） in egg yolk oil Ointment by HPLC. METHODS SunFire C 18 column was used and the mobile phase consisted ofacetonitrile-water （96∶ 4） at the flow rate of 1.0mL·min -1 .The wavelength was set at 292nm.Injection volume was 20μL.The column temperature was at 30℃. RESULTS The linear range of γ-tocopherol and α-tocopherol were 5.01～50.20μg·mL -1 and 50.82～508.75μg·mL -1 respectively.The average recoveries were 98.20% and 99.20%,with the RSD of 1.3%and 2.0%.The contents of γ-tocopherol and α-tocopherol in three batches of samples were 0.0231～0.0234mg·g -1 and 0.2586～0.2867mg·g -1 .CONCLUSION The method is simple and rapid,and suitable for thedetermination of Vitamin E （γ-tocopherol、α-tocopherol） in egg yolk oil Ointment.