半乳糖凝集素1,3在妊娠期高血压产妇胎盘中的表达及意义

Expressions and significance of galactose lectin family in placenta of patients with hypertensive disorder complicating pregnancy

目的探讨半乳糖凝集素1及3在妊娠期高血压疾病(HDCP)产妇胎盘中的表达及意义。方法选取2015年3月至2017年6月我院收治的70例HDCP产妇作为观察组,选取30例同期在我院体检的正常产妇作为对照组。采集产妇娩出后的胎盘组织,一份行免疫组织化学法检测半乳糖凝集素-1(Gal-1)和半乳糖凝集素-3(Gal-3)的表达,一份行蛋白印迹法检测Gal-1和Gal-3的蛋白水平。结果 (1)免疫组织化学检测显示:观察组产妇胎盘组织Gal-1和Gal-3表达明显高于对照组(P <0. 05),其中子痫前期产妇明显高于妊娠期高血压产妇和对照组(P <0. 05),而妊娠期高血压产妇又明显高于对照组(P <0. 05)。(2)蛋白印迹法检测显示:子痫前期产妇胎盘组织Gal-1和Gal-3蛋白水平明显高于妊娠期高血压产妇和对照组产妇(P <0. 05),而妊娠期高血压产妇又明显高于对照组产妇(P <0. 05)。结论 Gal-1和Gal-3在HDCP产妇胎盘组织中的表达明显增加,提示二者可能参与HDCP的发生发展。

Objective To investigate the expressions and significance of galactose lectin family in placenta of patients with hypertensive disorder complicating pregnancy （HDCP）. Methods A total of 70 patients with HDCP treated in Fifth Hospital in Wuhan from March,2015 to June,2017 were selected as the observation group.At the same time,thirty normal pregnant women were selected as the control group.The placenta tissues were collected after delivering and the expressions of galectin-1 （Gal-1） and galectin-3 （Gal-3） were detected by immunohistochemistry and Western Blot assay,respectively. Results （1） Immunohistochemical staining showed that the expressions of Gal-1 and Gal-3 in placenta tissues in the observation group were higher than those in the control group,among which the expressions of Gal-1 and Gal-3 in the pregnant women with preeclampsia were significantly higher than those in the pregnant women with hypertension and the control group,while the expressions of Gal-1 and Gal-3 in the pregnant women with hypertension were higher than those in the control group.（2） Western Blot assay indicated that the levels of Gal-1 and Gal-3 protein in placenta tissues of pregnant women with preeclampsia were higher than those in the pregnant women with hypertension and the control group,while the levels of Gal-1 and Gal-3 protein in the pregnant women with hypertension were higher than those in the control group. Conclusion The expressions of Gal-1 and Gal-3 in placenta tissues of patients with HDCP are increased,suggesting that Gal-1 and Gal-3 might be involved in the occurrence and development of HDCP.