攀枝花车前草中总黄酮的提取及其含量

Extraction and Determination of the Content of Total Flavanonids in Plantago asiatica L. in Panzhihua

目的:提取并测定攀枝花车前草中总黄酮的含量。方法:选取超声波乙醇浸提法提取车前草中黄酮类化合物,正交试验得出黄酮类化合物的最佳提取条件,对提取的黄酮类化合物进行定性分析,采用紫外分光光度法测定其含量。结果:车前草总黄酮的最佳提取条件为70%乙醇浸泡36 h,超声时间35 min,测得车前草内总黄酮的含量为1. 54%,车前草根和叶的平均回收率分别为98. 95%和98. 18%。同一条件下,测得车前草根中总黄酮含量大于叶中总黄酮含量。结论:该方法简单易行、无污染,是提取车前草黄酮类物质的有效途径,也为攀枝花车前草的进一步开发和研究奠定了基础。

Objective To extract and determine the content of total flavonoids in Plantago asiatica L.in Panzhihua. Methods： The flavonoids extracted from Plantago asiatica L.by ultrasonic ethanol extraction and the optimal experimental conditions of the ethanol extraction extracted flavonoids from Plantago asiatica L.were obtained by the orthogonal experiment.The determination of the content of total flavanonids had been characterized by ultraviolet spectrophotometry. Results： The optimum conditions of extracting total flavonoids from Plantago asiatica L.were as follows：the solvent was 70% ethanol for 36 h, the optimum time was 35 min.The results showed the content determination of total flavonoids in Plantago asiatica L.1.53% and the average recoveries of Plantago asiatica L.roots and foliage was 98.95% and 98.18%, respectively.Under the same condition, the content of flavonoids in Plantago asiatica L.root was bigger than that in Plantago asiatica L.foliage. Conclusion： The method without any pollution, simple and rapid, provides a theoretical basis for further development and research foundation of Plantago asiatica L.