木聚糖酶降解蔗渣木聚糖最优条件研究

Optimal Conditions for Degrading Bagasse Xylan by Xylanase

蔗渣木聚糖酶解过程中,木聚糖酶添加量、酶解pH、温度、时间及因素间交互作用均对低聚木糖得率产生不同程度的影响。采用响应曲面设计方法,通过分析优化所选指标与低聚木糖得率间函数关系模型,得到最优酶解工艺参数为酶添加量3.68%、酶解pH5.33、时间6 h、温度47.31℃。理论低聚木糖得率85.32%,平均聚合度2.24。在酶添加量3.7%、酶解pH5.3、时间6 h、温度47℃实际验证下,低聚木糖得率为82.04%,略低于理论数值,平均聚合度2.28。

Xylanase addition amount, pH, temperature, time and their interactions have different degrees of impact on xy- looligosaccharide yield in the enzymolysis of bagasse xylan. In this study, response surface methodology was applied to analyze and optimize the modeling by fitting the relationship between the selected index and xylooligosaccharide yield. The resuits showed that a theoretical xylooligosaccharide yield of 85.32% with average polymerization degree of 2.24 could be obtained under the optimal enzymolysis conditions： xylanase addition amount 3.68%, pH 5.33, time 6 h, temperature 47.31℃. An actual lower xylooligosaccharide yield of 82.04% with average polymerization degree of 2.28 was achieved under the enzymolysis conditions： xylanase addition amount 3.7%, pH 5.3, time 6 h and temperature 47 ℃.