乳腺癌患者外周血Cath-D、MUC1检测及其临床意义

Detection of Cath-D and MUC1 in peripheral blood of patients with breast cancer and its clinical significance

目的:探讨乳腺癌患者外周血组织蛋白酶D(Cath-D)、粘蛋白1(MUC1)检测及其临床意义。方法:选取185例乳腺癌患者作为观察组,选取健康者185例作为对照组,用逆转录聚合酶链反应RT-PCR技术检测Cath-D mR-NA和MUC1 mRNA,分析表达水平及临床意义。结果:观察组患者的Cath-D及MUC1检测阳性率高于对照组(P <0. 05)。Cath-D mRNA、MUC1 mRNA的阳性率与年龄、月经状况、雌激素受体(estrogen receptor,ER)、孕激素受体(pro-gesterone receptor,PR)孕激素受体(progesterone receptor,PR)相关。结论:检测Cath-D、MUC1可作为乳腺癌诊断的有效参考指标。

Objective： To explore the detection of Cathepsin D （Cath-D） and mucoprotein1 （MUC1） in peripheral blood of patients with breast cancer and its clinical significance. Methods： 185 patients with breast cancer were selected as the observation group and 185 healthy persons were selected as the control group. Cath-D mRNA and MUC1 mRNA were detected by reverse transcription polymerase chain reaction （RT-PCR）, and their expression levels and clinical significance were analyzed. Results： The positive rate of Cath-D and MUC1 in the observation group was higher than that in the control group （ P 〈0.05）. The positive rate of Cath-D mRNA and MUC1 mRNA was related to age, menstruation, estrogen receptor （ER） and （progesterone receptor） PR. Conclusion： Detection of Cath-D and MUC1 can be used as an effective reference index for breast cancer diagnosis.