多粘类芽孢杆菌(Paenibacillus polymyxa)XZ-2发酵条件优化的研究

Research on Optimization of Fermentation Conditions for Paenibacillus polymyxa Strain XZ-2

采用单因素实验和正交实验相结合的方法,对多粘类芽孢杆菌菌株XZ-2的培养基配方及发酵培养条件进行了优化研究。实验结果表明:该菌株的最佳培养基配方为蔗糖15 g/L,蛋白胨15 g/L,硫酸镁5 g/L,氯化钙3 g/L;最佳培养条件为初始pH值8,发酵温度30℃,发酵时间24 h,装液量80 mL/250 mL,接种量3%(体积分数),转速180r/min。

Single-factor experiment and orthogonal experiment were conducted to optimize the media components and fermentation conditions for Paenibacillus polymyxa strain XZ-2. The experimental results showed that： the best media components for XZ-2 were 15 g/L sucrose, 15 g/L peptone, 5 g/L magnesium sulfate, and 3 g/L calcium chloride; the optimum fermentation conditions for this strain were initial pH-value of medium at 8, fermentation temperature at 30 ℃, fermentation time for 24 h, liquid-loading volume 80 mL/250 mL, inoculum size 3% （volume fraction）, and rotate speed at 180 r/min.