硫酸镁联合拉贝洛尔对重度子痫前期患者胎盘中凋亡侵袭分子表达及母体内皮功能的影响

Effect of magnesium sulfate combined with labetalol on the apoptosis and invasion molecule expression in placenta as well as maternal endothelial function of patients with severe preeclampsia

目的:探讨硫酸镁联合拉贝洛尔对重度子痫前期患者胎盘中凋亡侵袭分子表达及母体内皮功能的影响。方法:重度子痫前期患者68例经随机数表法分为研究组、对照组各34例。对照组在常规治疗基础上加入硫酸镁治疗,研究组患者在常规治疗基础上加入硫酸镁联合拉贝洛尔治疗。对比两组胎盘组织中凋亡、侵袭分子表达量及母体内皮功能指标含量的差异。结果:研究组胎盘组织中Fas、Bax、Caspase-3mRNA的表达量低于对照组,Bcl-2mRNA的表达量高于对照组;胎盘组织中GPR30、LAMA4、MEST mRNA的表达量低于对照组,CDKN1CmRNA的表达量高于对照组;分娩前24h,研究组母体血清中ET-1、vWF的含量低于对照组,NO的含量高于对照组(P<0.05)。结论:重度子痫前期患者接受硫酸镁联合拉贝洛尔治疗,可有效抑制胎盘滋养细胞凋亡并增强其侵袭能力,同时改善母体内皮功能。

Objective：To investigate the effect of magnesium sulfate combined with labetalol on the apoptosis and invasion molecule expression in placenta as well as maternal endothelial function of patients with severe preeclampsia. Methods： A total of 68 patients with severe preeclampsia were divided into study group （n=34） and control group （n=34） by random number ta ble method. Control group received magnesium sulfate therapy on the basis of conventional therapy, and study group received magnesium sulfate combined with labetalol therapy on the basis of conventional therapy. The differences in the expression of apoptosis and invasion molecules in placenta tissue as well as the levels of maternal endothelial function indexes were compared between the two groups. Results： Fas, Bax and Caspase 3 mRNA expression in the placenta tissues of study group were lower than those of control group whereas Bcl-2 mRNA expression was higher than that of control group （P〈0.05）; OPR30, LAMA4 and MEST mRNA expression in placenta tissues were lower than those of control group whereas CDKN1C mRNA expression was higher than that of control group （P〈0.05） ; 24 h before delivery, maternal serum ET-1 and vWF levels of study group were lower than those of control group whereas NO level was higher than that of control group （P〈0.05）. Conclusion Magnesium sulfate combined with labetalol therapy can effectively inhibit the apoptosis of placental trophoblast cells and en hance their invasion ability, and also improve the maternal endothelial function in patients with severe preeclampsia.