肝癌患者血清中GPC3和GP73含量的变化及其与血管新生、上皮间质转化的相关性

Changes of serum GPC3 and GP73 contents in patients with liver cancer and their correlation with angiogenesis and epithelial mesenchymal transition

目的:研究肝癌患者血清中磷酯酰肌醇蛋白聚糖-3(GPC3)和高尔基体糖蛋白-73(GP73)含量的变化及其与血管新生、上皮间质转化的相关性。方法:选择在我院接受手术切除的肝癌患者作为肝癌组、同期体检的健康者作为对照组,测定两组受试者血清中GPC3、GP73的含量以及肝癌病灶、癌旁病灶内GPC3及GP73的含量、血管新生基因及上皮间质转化基因的表达量。结果:肝癌组患者血清中GPC3、GP73的含量显著高于对照组,肝癌病灶中GPC3、GP73的含量显著高于癌旁病灶且肝癌组患者血清中GPC3、GP73的含量与肝癌病灶中GPC3、GP73的含量呈正相关;肝癌病灶中SULT2B1、VEGF、Ang-2、Shh、Twist、N-cadherin的mRNA表达量显著高于癌旁病灶且与血清中GPC3、GP73的含量呈正相关,TUM5、Foxg1、E-cadherin的mRNA表达量显著低于癌旁病灶且与血清中GPC3、GP73的含量呈负相关。结论:肝癌患者血清中GPC3和GP73含量的升高与病灶内血管新生、上皮间质转化的加剧密切相关。

Objective： To study the changes of serum phosphatidylinositol proteinglycan-3 （GPC3） and Golgi protein-73 （GP73） contents in patients with liver cancer and their correlation with angiogenesis and epithelial mesenchymal transition. Methods： The patients with liver cancer who underwent surgical resection in our hospital between April 2014 and October 2017 were selected as liver cancer group, healthy subjects who received physical examination during the same period were selected as control group, and the contents of GPC3 and GP73 in serum as well as the contents of GPC3 and GP73, the expression of angiogenesis genes and epithelial mesenchymal transition genes in liver cancer lesions and adjacent lesions of the two groups of subjects were determined, Results： GPC3 and GP73 contents in serum of liver cancer group were significantly higher than those of control group（P〈0.05） , GPC3 and GP73 contents in liver cancer lesions were significantly higher than those in adjacent lesions（P〈0.05）, and the GPC3 and GP73 contents in serum of liver cancer group were positively correlated with GPC3 and GP73 contents in liver cancer lesions; SULT2B1 , VEGF, Ang-2, Shh, Twist and N- cadherin mRNA expression in liver cancer lesions were significantly higher than those in adjacent lesions（P〈0.05） and positively correlated with the GPC3 and GP73 contents in serum whereas TUM5, Foxgl and E cadherin mRNA expression were significantly lower than those in adjacent lcsions（P 〈0.05）and negatively correlated with the GPC3 and GP73 contents in serum. Conclusion： The increase of serum GPC3 and 0P73 contents in patients with liver cancer is closely related to the aggravation of angiogcncsis and epithelial mescnchymal transition in the lesions.