B细胞特异性莫洛氏鼠白血病病毒插入位点-1、原癌基因和肝癌缺失基因-1在宫颈癌中的表达及其临床意义

Expression and clinical significance of B-cell-specific Moloney leukemia virus insertion site-1,proto-oncogene and deleted in liver cancer-1 in cervical cancer

目的研究B细胞特异性莫洛氏鼠白血病病毒插入位点-1(BMI-1)、原癌基因(C-myc)和肝癌缺失基因-1(DLC-1)在宫颈癌中的表达及其意义。方法收集43例宫颈癌组织为A组,21例宫颈上皮内瘤变组织为B组,以及20例良性病变宫颈组织为C组。用免疫组化PV-9000二步法检测BMI-1、Cmyc和DLC-1蛋白的表达,用实时荧光定量聚合酶链式反应(RT-PCR)法检测BMI-1、C-myc和DLC-1 mRNA的表达,并分析其与宫颈癌临床病理因素的关系和三者表达的相关性。结果 A组、B组和C组BMI-1 mRNA的表达量分别为0. 93±0. 09,0. 29±0. 06和0. 17±0. 03,C-myc mRNA的表达量分别为0. 96±0. 08,0. 29±0. 04和0. 16±0. 03,DLC-1 mRNA的表达量分别为0. 11±0. 01,0. 83±0. 11和0. 95±0. 16,两两比较,差异均有统计学意义(均P <0. 05)。宫颈癌组织中BMI-1、C-myc和DLC-1的表达与患者的年龄、临床病理类型均无明显的相关性(均P> 0. 05),与患者的FIGO临床分期、淋巴结是否转移和分化程度均有明显的相关性(均P <0. 05)。DLC-1的蛋白表达水平与BMI-1、C-myc的蛋白表达水平均呈负相关(均P <0. 01)。结论在宫颈癌中,BMI-1和C-myc呈高表达,DLC-1呈低表达,其均与宫颈癌患者的FIGO分期、分化程度和淋巴结转移密切相关,联合检测BMI-1、C-myc和DLC-1可作为评价宫颈癌生物学行为和判断预后的参考指标。

Objective To study the expression and significance of B - cell - specific Moloney leukemia virus insertion site - 1 （ BMI - 1 ） , proto - oncogene （ C -myc） and deleted in liver cancer - 1 （ DLC - 1 ） in cervical cancer. Methods A total of 43 cases of cervical cancer were collected as group A, 21 cases of cervical intraepithelial neoplasia as group B and 20 cases of benign lesions as group C. The expression of BMI- 1, C -myc and DLC -1 protein was detected by immuno- histochemical PV -9000 two step method. The expression of BMI - 1, C - myc and DLC - 1 mRNA was detected by real time polymerase chain reaction （RT- PCR）, and the relationship with the clinicopathological factors of cervical cancer and the correlation between them were ana- lyzed. Results The expression levels of BMI - 1 mRNA in group A, group B and group C were respectively （0. 93 ±0.09）, （0. 29 ± 0. 06 ）and （0. 17 ＋ 0. 03 ）, the difference was statistically significant （ P 〈 0. 05 ）. The expression levels of C - myc mRNA in group A, group B and C group were （0. 96 ±0. 08）, （0. 29 ±0. 04） and （0. 16 ±0. 03）, and the difference was sta- tistically significant （ P 〈 0. 05 ）. The expression levels of DLC - 1 mRNA in group A, group B and group C were respectively （0. 11±0.01）, （0. 83 ±0. 11） and （0.95 ＋0. 16）, the difference was statistically significant （P 〈0.05）. There was no significant difference in the expression of BMI - 1, C - myc and DLC - 1 in the tissues of cervical cancer with the age and clinicopathological types of the patients （ all P 〉 0. 05 ）. There were significant differences in the clini- cal staging, lymph node metastasis and diffe-rentiation of FIGO （ all P 〈 0. 05 ）. The positive expression rate of BMI - 1 and C- myc increased with the clinical stage and differentiation degree of tumor. The positive expression rate of DLC -1 gradually decreased along with the clinical stage and differentiation degree of tumor. There was a negative correlation between the expression of DLC , 1 and the expression of BMI - 1 and C - myc protein （ all P 〈 0. 01 ）. Conclusion BMI - 1 and C - myc were highly expressed in cervical carcinoma and low expression of DLC - 1, all of them are closely related to FIGO stage, the degree of differentiation and lymph node metastasis of cervical cancer pa- tients. The combined detection of BMI - 1, C - myc and DLC - 1 can be used as a reference index to evaluate the biological behavior and oro-nosis of cervical cancer.