ATF5调节乳腺肿瘤细胞的生长和侵袭性的机制研究

ATF5 in regulating the growth and invasiveness of breast tumor cells

目的研究ATF5调节乳腺肿瘤细胞的生长和侵袭性的机制。方法在T47D乳腺癌细胞中,siRNA1组转染ATF5 siRNA1序列,siRNA2组转染siRNA1序列,对照组转染ATF5对照序列。用实时荧光定量聚合酶链式反应(PCR)检测ATF5 mRNA表达水平,用Western blot法检测ATF5蛋白表达,用克隆形成法检测细胞存活率,用细胞计数法(CCK-8)检测细胞增殖能力。结果转染24,48,72 h后,siRNA1组与siRNA2组细胞中ATF5表达水平均低于对照组(P <0. 05)。siRNA1组在24,48,72 h的生长速度比对照组低(P <0. 05)。siRNA1组、siRNA2组与对照组细胞的存活率分别为0. 98±0. 02,0. 98±0. 03和0. 98±0. 02,差异无统计学意义(P> 0. 05)。结论 ATF5表达下降能减少乳腺肿瘤细胞的生长和侵袭性。

Objective To investigate the mechanism of ATF5 regulate the growth and invasiveness of breast tumor cells. Methods The T47D breast cancer cells was used, siRNA1 group was transfeeted ATF5 siRNA1 sequence, siRNA2 group was transfected siRNA1 sequence, control group was transfected ATF5 control sequence. The expression of ATF5 mRNA were detected by real -time polymerase chain reaction （PCR）, the expression of ATF5 protein were detected by Western blot, the survival rate of cell Were detected by clones, and the proliferation ability of the cells were detected by cell count kit （CCK） - 8. Results After transfection of 24, 48 and 72 h, the expression of ATF5 in siRNA1 group and siRNA2 group were lower than that in control group （P 〈0. 05）. The growth rate of group siRNA1 at 24, 48 and 72 h were significantly lower than that of control group （ P 〈 O. 05 ）. The survival rates of siRNA1, siRNA2, control groups were 0. 98±0. 02, 0. 98±0. 03 and 0.98 ±0. 02, and there were no significant difference compared among three groups （P 〉 0. 05 ）. Conclusion The decreased expression of ATF5 can reduce the growth and invasiveness of breast cancer ceils and block the cell cycle.