神经生长因子及受体与早期宫颈癌嗜神经侵袭的相关性

Correlation of Nerve Growth Factor and Its Receptors Expression with Perineural Invasion in Early-stage Cervical Cancer

目的探讨神经生长因子(NGF)及其受体与早期宫颈癌嗜神经侵袭(PNI)和其他临床病理因素的相关性。方法选取2010年1月至2014年6月间,在广西医科大学附属肿瘤医院妇瘤科行根治性子宫切除术+盆腔淋巴结清扫术治疗的Ⅰb～Ⅱa2期宫颈癌患者60例,回顾性分析其临床病理资料,并用免疫组织化学法分析术后标本中NGF、TrkA、p75的表达情况,评估NGF及受体与PNI、其他病理因素的关联性。结果宫颈癌PNI的发生与NGF和受体TrkA的高表达明显正相关(P=0.025,P=0.011);PNI阳性的患者中,NGF、TrkA染色阳性面积也较PNI阴性的患者明显增多,差异有统计学意义(P=0.016, P=0.026);NGF、TrkA的表达也与淋巴脉管间隙浸润(LVSI)的发生密切相关(P=0.029, P=0.019),而与其他临床病理因素无相关性(均P>0.05);p75的表达与宫颈癌PNI和其他病理因素无明显关联(P>0.05)。结论 NGF、TrkA的高表达与早期宫颈癌PNI的发生具有一定的关联性,提示NGF和TrkA可能参与了PNI发生的相关分子机制。

Objective To evaluate the potential association of nerve growth factor（NGF） and its receptors TrkA and p75 NRT with perineural invasion（PNI） and other clinicopathological features of early-stage cervical cancer. Methods We retrospectively analyzed the clinicopathological data of 60 patients with stage Ⅰb-Ⅱa2 cervical cancer treated with radical hysterectomy plus pelvic lymphadenectomy in the Affiliated Tumor Hospital, Guangxi Medical University from January 2010 to June 2014. Immunohistochemistry（IHC） was performed on surgical samples to analyze the expression of NGF, TrkA and p75; the correlation of their expression with perineural invasion and other clinicopathological features was analyzed. Results Perineural invasion was correlated strongly with the high expression of NGF（P=0.025） and TrkA（P=0.011）. The rate of NGF-positive area and TrkA-positive area were significantly larger in PNI-positive patients than those in PNI-negative patients（P=0.016, 0.026, respectively）. The expressions of NGF and TrkA were significantly associated with lymphvascular space invasion（LVSI）（P=0.029, 0.019, respectively）, but not associated with other clinicopathological features（P〉0.05）. While p75 expression was not related to either perineural invasion or other clinicopathological features（P〉0.05）. Conclusion High expressions of NGF and TrkA are associated with perineural invasion, which suggests that NGF and TrkA may participate in the related molecular mechanism of PNI.