lncRNA在癫痫患者外周血液中的异常表达

Aberrant expression of lncRNA in peripheral blood mononuclear cells of epilepsy

目的：检测长链非编码核糖核酸（long non-coding RNA，IncRNA）在癫痫患者外周血液中的异常表达，探究lncRNA能否作为癫痫的生物标记物。方法：采用lncRNA芯片，抽取癫痫患者和正常健康对照者（各5例）的外周血样本，分离出单核细胞（PBMC〉并初步检测表达的lncRNA，寻找两组间表达异常的lncRNA。再根据结果，进一步扩大样本量，选择其中10个显著表达异常的lncRNA在58例癫痫患者和58例正常健康对照者中采用实时荧光定量（qRT-PCR）方法来验证已经筛查出的这10个lncRNA的相对表达水平。结果：病例组血浆NONHSAG012869（PR3）、NONHSAT006265（PR4）、ENST00000581634（PR6）和ENST00000524610（PR8）表达水平分别为2．99±1．59、2.29±0．77、4．24±1．51和11．08±2．24，较对照组的3．74±1.28、2．87±0．90、4.65±0.99、12．00±1．95显著下调（P〈0．05～0．001）。ROC曲线分析显示，4种lncRNA作为联合生物标记物对癫痫具有较高诊断价值（AUC为0．712，敏感度和特异度分别为33．8％和90．6％）；Logistic回归分析显示PR8具有最高相对危险度（Odd Ratio为2．459）。结论：PR3、PR4、PR6和PR8可能成为癫痫的联合生物标记物。

Objective：To explore the expression level of long non-coding RNA （lncRNA） in an effort to validate lncRNA as specific epilepsy biomarkers. Methods：The expression of lncRNAs in peripheral blood mononuclear cells （PBMCs） was measured by Agilent Human lncRNA array. 10 lncRNAs were chosen for further validation with quantitative real-time reverse transcription polymerase chain reaction （qRT-PCR） in larger samples including 58 cases and 58 controls, Results：THE expressed levels of NONHSAG012869 （PR3）, NONHSAT006265 （PR4）, ENST00000581634 （PR6） and ENST00000524610 （PR8） in patients with epilepsy were 2.99±1.59,2.29±0.77, 4. 24±1.51,11.08±2.24 respectively, significantly （P〈0.05 - 0.001）, lower than those of the control group（3.74 ± 1.28,2. 87 ± 0. 90,4.65 ± 0. 99 and 12.00±1.95 respectively. ROC curves analysis revealed that the four lncRNAs combined as a panel had significantly diagnostic value in differentiating epilepsy （AUC was 0. 712, sensitivity and specificity were 33.8% and 90.60% respectively）; Logistic regression analysis demonstrated that PR7 had the greatest relative risk against epilepsy （Odd Ratio was 2.459）. Conclusion：PR3, PR4, PR6 and PR8 may serve as specific biomarkers for diagnosis of epilepsy.