鸢尾素对胆管癌Hucct-1细胞增殖、凋亡、迁移和侵袭的影响

Effects of Irisin on proliferation, apoptosis, migration and invasion of human cholangiocarcinoma cell line Hucct-1

目的探讨鸢尾素（Irisin）对胆管癌Hucct-1细胞增殖、凋亡、迁移和侵袭的影响。方法使用Irisin处理细胞后，采用CCK-8实验和流式细胞实验检测Irisin对胆管癌细胞增殖及凋亡影响；采用划痕实验和Transwell侵袭实验检测Irisin对胆管癌细胞迁移和侵袭能力的影响；采用Westernblot实验检测胆管癌细胞E-cadherin、N-cadherin、Vimentin的表达。结果CCK-8实验结果显示，Irisin以剂量依赖的方式抑制胆管癌细胞的增殖。细胞凋亡检测结果显示，Irisin组细胞凋亡率[（14．8±0．9）％]明显高于对照组[（5．4±0．6）％]，差异有统计学意义（P〈0．05）。划痕实验结果显示，Irisin组细胞划痕愈合率[（15±1）％[明显低于对照组[（28．0±2．0）％]，差异有统计学意义（P〈0．05）。Transwell侵袭实验结果显示，Irisin组细胞数（96．0±7．0）明显低于对照组（155．0±9．0），差异有统计学意义（P〈0．05）。Westernblot实验显示，Irisin处理之后，细胞中E-cadherin表达水平上升，N-cadherin、Vimentin表达水平下降。结论Irisin能够抑制胆管癌细胞增殖、促进其凋亡，同时可以抑制胆管癌细胞的迁移及侵袭。

Objective To investigate the effect of Irisin on proliferation, apoptosis, migration and invasion of human cholangioeareinoma cell line Hueet- 1. Methods After treatment with Irisin, CCK-8 assay and flow cytometry assay were conducted to investigate the effect of Irisin on proliferation and apoptosis of cholangiocarcinoma cells. Scratch test and transwell invasion assay were used to studythe effect of Irisin on the migration and invasion ability of cholangiocarcinoma cells. Western blot was utilized to detect the expression of E-cadherin, N-cadherin and Vimentin in cholangiocarcinoma cells. Results CCK-8 assay showed that Irisin inhibited cholangiocarcinoma cell proliferationin a dose- dependent manner. Flow eytometry assay showed that the apoptosis rate of Irisin group [（14.8±0.9）%] was higher than that in the control group [（5.4±0.6）%], （P〈0.05）. The scratch test showed that the rate of cell scratch healing in Irisin group [（15.0±1.0）%] was significantly lower than that in the control group [（28.0±2.0）%] （P〈0.05）. Transwell invasion test showed that the number of cells in Irisin group was （96.0±7.0）, which was significantly lower than that in control group （155.0±9.0） （P〈0.05）. Western blot showed that the expression of E-cadherin increased and N- cadherin and Vimentin decreased after Irisin treatment. Conclusion Irisin inhibits proliferation, migration and invasion and promote apoptosis of cholangiocareinoma cells.