恩诺沙星在水生生物体内代谢产物的分析

Analysis on the Metabolites of Enrofloxacin in Aquatic Organism

抗生素因有高效、广谱的特点而被广泛使用。由于这类化合物具有亲水、弱挥发性等特征,使得天然水体可能成为其存在的主要"汇",进而对水生生物体造成一定的毒害效应。该研究以鲤鱼(Cyprinus carpio)为受试生物,以恩诺沙星为给药化合物,采用高效液相色谱串联三重四级杆质谱仪(HPLC-MS/MS),在电喷雾离子源正离子模式下进行选择离子(SIR)扫描,分析了恩诺沙星在鲤鱼肝脏中的代谢产物及转化路径。实验结果表明,在肝脏中共鉴定出8种代谢产物,包括脱乙基产物(M1)、哌嗪环降解产物(M2)、脱乙炔产物(M3)、脱氟产物(M4)、羟基化产物(M5、M8)、甲酰化产物(M6)和甲基化产物(M7),其中,脱乙基生成环丙沙星为主要物质。该研究结果为深入了解恩诺沙星在水生生物体内的生物转化提供了理论基础。

Antibiotics are efficient and broad-spectrum which make them widely used in human medicine, animal husband- ry, agriculture and aquaculture. Due to their hydrophilicity and weak volatility, natural water may become the main ＂sink＂, which will cause toxic effects on aquatic organisms. In this study, carp （Cyprinus carpio） was used as the test organism, and enrofloxacin was administered as the compound. High performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） coupled with electrospray ionization source operated in positive mode for SIR scan was used to identify the metabolites and metabolic pathway of enrofloxacin in carp liver. The results showed that 8 metabolites were found, including products of desethylation （M1）, piperazine ring cleavage （M2）, de-acetylene （M3）, defluorination （M4）, hydroxylation （MS, M8）, formylation （M6） and methylation （MT）, in which desethylation product ciprofloxacin was the main metabolite. And this study provides theoretical basis for further understanding the biotransformation of eurofloxacin in aquatic organisms.