组织HIF-1α表达与子宫内膜癌化疗后远期预后的关系

Relationship between tissue HIF-1α and long-term prognosis of endometrial cancer after chemotherapy

目的探讨子宫内膜癌中缺氧诱导因子-1α(HIF-1α)的表达与患者化疗后远期预后的关系。方法采用免疫组化法分别检测62例子宫内膜癌、30例不典型增生子宫内膜以及30例正常子宫内膜组织中的HIF-1α表达情况,并收集所有患者的临床病理资料予以对比分析。此外,对62例子宫内膜癌患者予以化疗,并进行为期5年的随访,分析HIF-1α表达与患者复发及死亡情况的关系。结果 HIF-1α在子宫内膜癌组织中的阳性表达率为67.74%(42/62),高于不典型增生子宫内膜组织以及正常子宫内膜组织中的26.67%(8/30)、0.00%(0/30),而不典型增生子宫内膜组织中HIF-1α阳性表达率又高于正常子宫内膜组织,差异均有统计学意义(P<0.05)。子宫内膜癌的HIF-1α表达阳性率在不同分期、分化程度、浸润深度、淋巴结转移中差异均有统计学意义(P<0.05)。复发组患者子宫内膜组织中HIF-1α表达阳性率为89.28%(25/28),高于未复发组的50.00%(17/34),差异有统计学意义(P<0.05)。生存组患者子宫内膜组织中HIF-1α表达阳性率为43.48%(10/23),低于死亡组的82.05%(32/39),差异有统计学意义(P<0.05)。结论 HIF-1α的高表达与子宫内膜癌患者病情严重程度、局部侵袭以及淋巴结转移有关,其水平的高表达可提示早期复发以及死亡。

Objective To investigate the relationship between the expression of hypoxia-inducible factor-1α（HIF-1α）in endometrial cancer and long-term prognosis after chemotherapy. Methods Immunohistochemistry was used to detect the expression of HIF-1α in 62 cases of endometrial carcinoma,30 cases of dysplasia endometrium and 30 cases of normal endometrium. The clinical and pathological data of all patients were collected and compared. In addition,62 patients with endometrial cancer were treated with chemotherapy for a 5-year follow-up,and the relationship between HIF-1α expression and recurrence and mortality was analyzed. Results The positive expression rate of HIF-1α in endometrial carcinoma tissues was 67.74%（42/62）,which was higher than that in dysplasia endometrial tissues and normal endometrial tissues[26.67%（8/30）,0.00%（0/30）],the positive expression rate of HIF-1α in uterine endometrial tissue was higher than that in normal endometrial tissue,and the difference was statistically significant（P〈0.05）. The positive rate of HIF-1α expression in endometrial carcinoma was statistically significant in different stages,differentiation,depth of invasion and lymph node metastasis（P〈0.05）. The positive rate of HIF-1α expression in the endometrium of the recurrent group was 89.28%（25/28）,which was higher than that of the non-recurrent group（50.00%,17/34）. The difference was statistically significant（P〈0.05）.The positive rate of HIF-1α expression in the endometrium of the survival group was 43.48%（10/23）,which was lower than that of the death group（82.05%,32/39）. The difference was statistically significant（P〈0.05）. Conclusion The high expression of HIF-1α was associated with severity of disease,local invasion,and lymph node metastasis in patients with endometrial cancer,and high levels of expression could be used to suggest early relapse and death.