参附注射液对大鼠脑缺血再灌注损伤的保护作用研究

Protective effects of shenfu injection on cerebral ischemia reperfusion injury in rats

目的研究参附注射液对大鼠脑缺血再灌注损伤的保护作用。方法雄性SD大鼠120只,320～350 g,按随机数字表法分为假手术组、模型对照组、尼莫地平注射液组、参附注射液低、中、高剂量组,每组20只,每天给药1次,于手术前给药7 d,给药5 d后采用线栓法建立脑缺血模型,缺血2 h后再灌注24 h。假手术组除不进行颈动脉结扎及插线处理外,其余操作与模型组相同。灌注24 h后进行神经功能学评分,腹主动脉取血检测血液流变学,取脑组织检测脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GHS)水平,对脑组织进行2,3,5-三苯基氯化四氮唑(TTC)染色计算脑梗死面积和脑组织病理学检查。结果与模型对照组比较,参附注射液中、高剂量组大鼠神经功能明显改善(P <0. 05),脑梗死面积百分比明显减少(P <0. 05);参附注射液高剂量组大鼠全血粘度值降低(P <0. 01);参附注射液中、高剂量组大鼠脑组织MDA水平明显降低(P <0. 01),SOD、GSH水平明显升高(P <0. 01),神经纤维肿胀、神经元变性、炎间质性水肿和炎症等脑组织病理变化明显改善。结论参附注射液对大鼠脑缺血再灌注模型有明显的保护作用。

Objective To study the protective effect of shenfu injection on cerebral ischemia reper- fusion injury in rats. Methods 120 male Sprague Dawley （SD） rats（320 -350 g） were randomly divided into sham operation group, model control group, Nimodipine injection group, low, medium and high dose group of shenfu injection according to gender weight. 20 males in each group were given medicine once a day for 7 days before operation. The cerebral ischemia model was established by thread embolization after 5 days of administration. In the sham operation group, the other operations were the same as those in the mod- el group except for carotid artery ligation and thread insertion. After 24 hours of perfusion, the neurological score, abdominal aorta blood flow, malondialdehyde （MDA） , superoxide dismutase （SOD） and glutathione （GHS） levels in brain tissues were measured. Triphenyltetrazolium chloride （TTC） staining was used to calculate the area of cerebral infarction and pathological examination of brain tissues. Results Compared with the model control group, the middle and high dosage of shenfu injection could obviously improve the nerve function and increase the percentage of cerebral infarction area （ P 〈 0. 05 ） ; the high dosage group of shenfu injection could obviously decrease the whole blood viscosity （P 〈 0. 01 ） ; the middle and high dosage of shenfu injection could obviously reduce the level of MDA in rat brain tissue （P 〈 0. 01 ） while increasing the levels of SOD and GSH （ P 〈0.01 ） ,finally could significantly improve the pathological changes of brain tissues such as mild swelling of nerve fibers, mild neuronal degeneration, inflammatory interstitial edema and inflammation. Conclusions Shenfu injection has obvious protective effect on cerebral ischemia reperfusion model in rats.