羊瘙痒因子263K感染仓鼠脑组织中兰尼碱受体2表达变化的研究

Association between prion infection and Ryanodine receptor 2 in scrapie-263 K infected hamster

目的探索RyR2在朊病毒感染金黄仓鼠脑组织中的表达变化。方法通过免疫组织化学染色观察到RyR2在正常仓鼠及263K毒株感染仓鼠的脑区分布情况；通过免疫印迹方法观察到正常及263K毒株感染仓鼠终末期脑组织中RyR2表达变化情况；通过免疫荧光双标染色检测RyR2是否与朊蛋白存在共定位关系。结果RyR2主要分布在仓鼠的小脑浦肯野细胞、大脑皮层，而在海马、下丘脑和嗅球也有表达。263K毒株感染仓鼠终末期脑组织中，RyR2表达显著下降；免疫荧光双标染色发现，RyR2与朊蛋白存在共定位关系。结论RyR2的表达在朊病毒感染的金黄仓鼠中变化明显，可能与认知功能的下降及神经元调亡有关。此研究为进一步探讨RyR2在朊病毒病中的作用和分子机制提供了一定基础。

Objective To classify the expression of RyR2 in the brain of prion infected hamsters. Methods Immunohistochemical assays was used to verify that the location of RyR2 in the brain slices of hamster. Assays of the brain samples of intracranial inoculation of scrapie infected hamster （ agent 263 K） and normal hamster were tested to evaluate the RyR2 expression by Western Blotting. Immunofluorescent assays were used to verify the co-location between RyR2 and PrP protein. Results RyR2 mainly located in cortex and Purkinje cells with parts of which are distributed in thalamus, hippocampus and olfactory bulb. The expression of RyR2 significantly decreased in the 263 K infected hamster at terminal stage. The Immunofluorescence tests showed that RyR2 was colocalized with PrP protein. Conclusions The experimental data showed that RyR2 may play a crucial role in prion disease, which might be closely linked to the cognition impairment and neuron loss. The relation between RyR2 and prion disease still needs further research.