一种肉桂水提物抗单纯疱疹病毒1型活性研究

Assessment of the anti-herpes simplex virus type 1 activity of a water extract from Cinnamon

目的评价一种肉桂水提物KPC—rg1对单纯疱疹病毒1型（herpes simplex virus type 1，HSV-1）的抗病毒活性，并探索其可能的广谱抗病毒作用。方法体外培养Vero细胞，以HSV-1和HSV-1与KPC-rg1共孵育混悬液分别感染细胞，观察细胞形态并检测病毒载量；利用小鼠和树鼢角膜感染模型对HSV-1和HSV-1与KPC-rg1共孵育混悬液分别感染动物后的角膜病变情况进行评分；通过细胞病变效应实验和荧光衰减实验测定KPC—rg1对9种病毒的体外抗病毒活性。结果被HSV-1感染的Vero细胞加入KPC—rg1（0．0001～1．0ms／ml）后对病毒复制有抑制作用，而HSV-1与KPC—rg1（0．001～1．0mg／ml）共孵育后能保护细胞不被感染；KPC—rg1对HSV-1感染的动物角膜病变有一定的改善作用，而KPC—rg1与HSV-1共孵育后能完全保护动物不被HSV-1感染；KPC—rg1对多种包膜病毒有潜在的广谱抗病毒作用。结论KPC—rg1是一种溶胶（丁达尔现象），可能与病毒能快速形成稳定的KPC-rg1／病毒超纳米颗粒结构，使病毒失去感染能力。KPC—rg1通过包裹HSV—1病毒抑制其感染，减少了潜伏或新增殖病毒对机体造成损伤，可缓解患者反复遭受病痛折磨。本研究丰富了肉桂水提物在抗病毒，特别是抗HSV-1的作用，为其开发成为潜在的抗HSV-1药物提供了初步科学依据。

Objective To evaluate the anti- herpes simplex virus type 1 （HSV-1） activity of KPC- rg1, a water extract from Cinnamomum cassia, and explore its potential function of broad-spectrum antivirus effect. Methods In vitro, the changes of morphology of Vero cells were assessed and viral loads were detected after cells were infected with HSV-1 alone and HSV-1 pre-treated with KPC-rgl respectively. The corneal lesions of mouse and tree shrew corneal infection model were evaluated after they were infected with HSV-1 alone and HSV-1 pre-treated with KPC-rgl respectively. The antiviral activity of KPC-rgl against 9 viruses were measured by CPE and GFP reduction assays. Results The virus replication of HSV-1 infected cells was moderately inhibited by KPC-rgl in a dose range of 0. 0001-1.0 mg/ml, while the cells were completely protected when they were infected with HSV-1 pre-treated with KPC-rgl （0. 001-1.0 mg/ml）. The corneal lesions of animals were improved in both mouse and tree shrews models infected with HSV-1 after the treatment of KPC-rg1, while animals were completely protected from infection when HSV-1 pretreated with KPC-rgl. KPC-rgl had a potential anti-virus effect on the enveloped viruses such as HSV-1, HCMV, RSV alone and HIV-1. Conclusions KPC-rgl is a collosol （Tyndall effect） which would immediately form a stable super-nanoparticle structure of KPC-rg1/virus when encounter virus, and thus the virus coated by KPC-rgl lost its ability of infection. KPC-rgl can reduce the suffering by newly or latent virus infection because its encapsulation of virus and inhibition of further infection. Our study added additional proofs of the anti-viral property of the water extract from Cinnamomum cassia, and provided a further basis to develop KPC-rgl as a drug which could be potentially applied in clinic to treat HSV-1 infection.