术前消癌平联合TEC新辅助化疗对乳腺癌细胞恶性生物学行为的影响

Effect of preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy on malignant biological behaviors of breast cancer cells

目的:研究术前消癌平联合TEC新辅助化疗对乳腺癌细胞恶性生物学行为的影响。方法:选择在本院接受新辅助化疗的乳腺癌患者作为研究对象,随机分为接受术前消癌平联合TEC新辅助化疗的联合组以及接受TEC新辅助化疗的对照组。手术切除后,取乳腺癌病灶并测定增殖基因、侵袭基因的mRNA表达量以及血管新生分子的蛋白含量。结果:手术切除后,联合组患者乳腺癌病灶内USP39、CyclinD1、c-myc、Sema4D、CatB、ADAM17、uPA的mRNA表达量以及VEGF、Ang-2、bFGF的蛋白含量显著低于对照组,TCEAL17、MFN2、TIMP1、E-cadherin的mRNA表达量以及MMRN2、PEDF的蛋白含量显著高于对照组。结论:乳腺癌术前消癌平联合TEC新辅助化疗能够较单用TEC新辅助化疗能够有效地抑制癌细胞增殖、侵袭及血管新生。

Objective： To study the effect of preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy on malignant biological behaviors of breast cancer cells. Methods： The patients with breast cancer who underwent neoadjuvant chemotherapy in Renshou County People’s Hospital between June 2014 and December 2017 were enrolled and randomly divided into the combined group who were given preoperative Xiaoaiping combined with TEC neoadjuvant chemotherapy and the control group underwent TEC neoadjuvant chemotherapy. After surgical resection, the breast cancer lesion was taken to determine the mRNA expression of proliferation genes and invasion genes as well as the protein levels of angiogenesis molecules. Results： After surgical resection, USP39, CyclinD1, c-myc, Sema4D, CatB, ADAM17 and uPA mRNA expression as well as VEGF, Ang-2 and bFGF protein levels in breast cancer lesions of combined group were significantly lower than those of control group, whereas TCEAL17, MFN2, TIMP1 and E-cadherin mRNA expression as well as MMRN2 and PEDF protein levels were significantly higher than those of control group. Conclusion： For patients with reast cancer, application of Xiaoaiping combined with TEC neoadjuvant chemotherapy before they underwent resection surgery can be more effective than TEC neoadjuvant chemotherapy alone to inhibit the proliferation, invasion and angiogenesis of cancer cells.